Water-soluble single molecular probe for simultaneous detection of viscosity and hydrazine

Hydrazine (N₂H₄) can cause serious damage to human health, while intracellular viscosity is highly associated with many diseases and cellular dysfunctions. Herein, we report the synthesis of a dual-responsive organic molecule-based fluorescent probe with excellent water solubility being capable of detection of N₂H₄ and viscosity through dual-fluorescence channels in "turn on" manner for both. Besides sensitive detection of N₂H₄ in aqueous solution with detection limit of 0.135 μM, this probe could be used for vapor N₂H₄ detection in colorimetric and fluorescent manners. In addition, the probe demonstrated viscosity-dependent fluorescence enhancement behavior, and as high as 150-fold enhancement could be obtained at 95% glycerol aqueous solution. Cell imaging experiment revealed that the probe could be used for the discriminating of living and dead cells.