Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hormones and Bone Tissue01:17

Hormones and Bone Tissue

2.8K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.8K
Bone Formation by Endochondral Ossification01:24

Bone Formation by Endochondral Ossification

4.9K
Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...
4.9K
Bone Remodeling01:40

Bone Remodeling

38.4K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.4K
Bone Formation by Intramembranous Ossification01:29

Bone Formation by Intramembranous Ossification

6.6K
Intramembranous ossification is one of the two processes involved in the development of bones within an embryo. The flat bones of the face, most of the cranial bones, and the clavicles are formed via this process. During intramembranous ossification, the bones develop directly from sheets of undifferentiated mesenchymal connective tissue.
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into ...
6.6K
Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

3.1K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.1K
Role of Vitamins in Maintaining Bone Health01:25

Role of Vitamins in Maintaining Bone Health

3.5K
The growth and maintenance of bone are regulated by a combination of nutritional factors, including vitamins, such as vitamin A, B12, C, D, and K.
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ADJUNCTIVE PROBIOTICS SHOW A POSITIVE TREND BUT INCONCLUSIVE CLINICAL BENEFIT IN PERI-IMPLANTITIS MANAGEMENT.

The journal of evidence-based dental practice·2025
Same author

The Incidence and Severity of Medication Related Osteonecrosis of the Jaws is Similar in Male and Female Mice.

Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons·2024
Same author

Next-generation 5G fusion-based intelligent health-monitoring platform for ethylene cracking furnace tube.

Mathematical biosciences and engineering : MBE·2022
Same author

Endoplasmic reticulum-targeted polymer dots encapsulated with ultrasonic synthesized near-infrared carbon nanodots and their application for in vivo monitoring of Cu<sup>2</sup>.

Journal of colloid and interface science·2022
Same author

The different effect of tongue motor task training (TTT) and strength training (ST) on the modulation of genioglossus corticomotor excitability and upper airway stability in rats.

Sleep·2022
Same author

Development of BET Inhibitors as Potential Treatments for Cancer: Optimization of Pharmacokinetic Properties.

ACS medicinal chemistry letters·2022

Related Experiment Video

Updated: Aug 8, 2025

Culturing and Measuring Fetal and Newborn Murine Long Bones
06:58

Culturing and Measuring Fetal and Newborn Murine Long Bones

Published on: April 26, 2019

8.3K

FBXO11 regulates bone development.

Hong Huang1, Jianrong Lu2, Ikramuddin Aukhil1

  • 1Department of Periodontology, University of Florida, Gainesville, FL 32610, USA.

Bone
|March 2, 2023
PubMed
Summary

FBXO11 regulates bone development by controlling osteoblast differentiation. Its deficiency leads to Snail1 accumulation, inhibiting bone formation and mineralization.

Keywords:
Bone formationFBXO11OsteoblastSnail1

More Related Videos

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
14:55

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Published on: June 24, 2018

9.3K
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

7.1K

Related Experiment Videos

Last Updated: Aug 8, 2025

Culturing and Measuring Fetal and Newborn Murine Long Bones
06:58

Culturing and Measuring Fetal and Newborn Murine Long Bones

Published on: April 26, 2019

8.3K
Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
14:55

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Published on: June 24, 2018

9.3K
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

7.1K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Developmental Biology

Background:

  • FBXO11 is a key component of the SKP1-cullin-F-boxes ubiquitin ligase complex.
  • The function of FBXO11 in bone development remains largely unknown.

Purpose of the Study:

  • To elucidate the role of FBXO11 in regulating osteogenic differentiation and bone development.
  • To investigate the molecular mechanism by which FBXO11 influences bone formation.

Main Methods:

  • FBXO11 gene knockdown and overexpression in mouse pre-osteoblast MC3T3-E1 cells.
  • Generation of osteoblastic-specific FBXO11 conditional knockout mouse models (Col1a1-ERT2-FBXO11KO and Bglap2-FBXO11KO).
  • Analysis of osteogenic and osteoclastic activity, Snail1 protein ubiquitination, and bone mineralization.

Main Results:

  • FBXO11 knockdown reduced osteogenic differentiation; overexpression accelerated it in vitro.
  • FBXO11 deficiency in conditional knockout mice inhibited bone growth with reduced osteogenic activity.
  • FBXO11 deficiency caused Snail1 protein accumulation in osteoblasts by reducing its ubiquitination, suppressing osteogenic activity and bone mineralization.

Conclusions:

  • FBXO11 plays a critical role in regulating osteoblast differentiation and bone formation.
  • FBXO11 deficiency inhibits bone development by promoting Snail1 accumulation, which impairs osteogenic activity and mineralization.