Characterization of Ca²⁺-ATPase, LMCA1, with native cell membrane nanoparticles system

Ca²⁺-ATPases are membrane pumps that transport calcium ions across the cell membrane and are dependent on ATP. The mechanism of Listeria monocytogenes Ca²⁺-ATPase (LMCA1) in its native environment remains incompletely understood. LMCA1 has been investigated biochemically and biophysically with detergents in the past. This study characterizes LMCA1 using the detergent-free Native Cell Membrane Nanoparticles (NCMNP) system. As demonstrated by ATPase activity assays, the NCMNP7-25 polymer is compatible with a broad pH range and Ca²⁺ ions. This result suggests that NCMNP7-25 may have a wider array of applications in membrane protein research.