Effects of medium-chain fatty acids (MCFAs) on in vitro rumen fermentation, methane production, and nutrient...

This experiment was conducted to investigate the effects of different dosages and types of medium-chain fatty acids (MCFAs) on rumen fermentation in vitro under low- and high-concentrate diets. For this purpose, two in vitro experiments (Exp.) were conducted. In Exp. 1, the concentrate-roughage ratio of the fermentation substrate [total mixed rations (TMR), dry matter (DM) basis] was 30:70 (low-concentrate diet), while in Exp. 2, it was 70:30 (high-concentrate diet). Three types of MCFAs with octanoic acid (C₈ ), capric acid (C₁₀ ), and lauric acid (C₁₂ ) were added accounting for 1.5%, 6%, 9%, and 15% of the in vitro fermentation substrate weight (200 mg or 1 g, DM basis) based on control group, respectively. The results showed that the addition of MCFAs all could significantly reduce methane (CH₄ ) production and the number of rumen protozoa, methanogens, and methanobrevibacter under the two diets with the dosages increased (p < 0.05). In addition, MCFAs had a certain degree of improvement on rumen fermentation and influenced in vitro digestibility under low- and high-concentrate diets, and their effects were related to the dosages and types of MCFAs. This study provided a theoretical basis for the selection of types and dosages of MCFAs in ruminants production.