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Updated: Aug 8, 2025
Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
Published on: June 2, 2021
Cytoplasmic dynein1 intermediate-chain2 regulates cellular trafficking and physiopathological development in
Lily Lin1,2, Ibrahim Tijjani1, Hengyuan Guo3
1Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Abstract:
The cytoplasmic dynein 1, a minus end-directed motor protein, is an essential microtubule-based molecular motor that mediates the movement of molecules to intracellular destinations in eukaryotes. However, the role of dynein in the pathogenesis of Magnaporthe oryzae is unknown. Here, we identified cytoplasmic dynein 1 intermediate-chain 2 genes in M. oryzae and functionally characterized it using genetic manipulations, and biochemical approaches. We observed that targeted the deletion of MoDYNC1I2 caused significant vegetative growth defects, abolished conidiation, and rendered the ΔModync1I2 strains non-pathogenic. Microscopic examinations revealed significant defects in microtubule network organization, nuclear positioning, and endocytosis ΔModync1I2 strains. MoDync1I2 is localized exclusively to microtubules during fungal developmental stages but co-localizes with the histone OsHis1 in plant nuclei upon infection. The exogenous expression of a histone gene, MoHis1, restored the homeostatic phenotypes of ΔModync1I2 strains but not pathogenicity. These findings could facilitate the development of dynein-directed remedies for managing the rice blast disease.
Insights
Cytoplasmic dynein 1 in Magnaporthe oryzae is crucial for fungal growth, development, and pathogenicity. Its deletion disrupts microtubule organization and leads to non-pathogenic strains, suggesting potential therapeutic targets for rice blast disease.
Area of Science:
- Molecular Mycology
- Cell Biology
- Plant Pathology
Background:
- Cytoplasmic dynein 1 is a vital microtubule motor protein in eukaryotes.
- Its function in Magnaporthe oryzae pathogenesis was previously uncharacterized.
Purpose of the Study:
- To investigate the role of cytoplasmic dynein 1 intermediate-chain 2 (MoDync1I2) in M. oryzae.
- To explore MoDync1I2's contribution to fungal development and pathogenicity.
Main Methods:
- Genetic manipulation (gene deletion) of MoDync1I2 in M. oryzae.
- Microscopic analysis of microtubule organization, nuclear positioning, and endocytosis.
- Biochemical approaches including protein localization studies.
Main Results:
- Deletion of MoDync1I2 caused severe defects in vegetative growth and conidiation.
- ΔModync1I2 strains exhibited abolished pathogenicity and defects in microtubule organization.
- MoDync1I2 localized to microtubules and co-localized with histone in plant nuclei during infection.
Conclusions:
- MoDync1I2 is essential for M. oryzae growth, development, and pathogenicity.
- Defects in MoDync1I2 impact microtubule organization and nuclear positioning.
- Dynein-directed strategies may offer new avenues for managing rice blast disease.
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