Exploratory integrated analysis of circulating exosomal miRNA and tissue mRNA related to long-term physical activity...

Physical activity exerts various positive effects on both physical and mental health. Although the comprehensive expression profiles of each microRNA (miRNA) or messenger RNA (mRNA) related to physical activity have already been reported, the association between miRNA and mRNA remains unclear. Here, the integrated study was conducted to comprehensively explore the potential miRNA-mRNA relationships related to long-term physical activity over 25 years. Genome-wide public deposited mRNA expression data of adipose tissue (GSE20536) from six same-sex twin pairs (no information regarding gender) and of skeletal muscle tissue (GSE20319) from ten same-sex twin pairs (four female twin pairs) were used, and differentially expressed mRNAs (DEMs) related to discordant leisure-time physical activity for 30 years were identified using GEO2R. Overlapped mRNAs between DEMs and predicted possible target mRNAs, based on a previous study and TargetScan tool, were then identified and used as long-term physical activity-related mRNAs targeted by miRNAs. In adipose tissue, 36 mRNAs and 42 mRNAs were identified as upregulated or downregulated DEMs, respectively. Based on the results of the overlapped analysis between DEMs and predicted possible target mRNAs targeted by miRNAs, 15 upregulated mRNAs, including NDRG4, FAM13A, ST3GAL6, and AFF1, and 10 downregulated mRNAs, including RPL14, LBP, and GLRX, were identified. In muscle tissue, three downregulated mRNAs overlapped with the predicted target mRNAs targeted by miRNAs. Fifteen upregulated mRNAs in adipose tissue showed a tendency to enrich in "Cardiovascular" in GAD_DISEASE_CLASS category. Potential miRNA-mRNA relationships related to long-term physical activity over 25 years were identified through bioinformatics analysis.