MAPKAPK5-AS1/miR-515-5p/CAB39 Axis Contributes to Non-small Cell Lung Cancer Cell Proliferation and Migration
Yueming Zhao1, Danyang Zhou1, Yuan Yuan1
1Department of Respiratory, Nanjing First Hospital, Qinhuai District, No.68, Changle Road, Nanjing, 210012, Jiangsu, China.
Molecular Biotechnology
|March 3, 2023
Summary
Long noncoding RNA MAPKAPK5-AS1 promotes non-small cell lung cancer (NSCLC) progression by upregulating CAB39 via sponging miR-515-5p. This finding offers potential therapeutic targets for NSCLC treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long noncoding RNAs (lncRNAs) are implicated in human carcinoma development.
- The role of lncRNA MAPKAPK5 antisense RNA 1 (MAPKAPK5-AS1) in non-small cell lung cancer (NSCLC) remains largely unknown.
- MAPKAPK5-AS1 has demonstrated oncogenic functions in other cancers, such as colorectal cancer.
Purpose of the Study:
- To investigate the regulatory role of MAPKAPK5-AS1 in NSCLC progression.
- To elucidate the molecular mechanism underlying MAPKAPK5-AS1's function in NSCLC.
- To identify potential therapeutic targets for NSCLC based on MAPKAPK5-AS1's pathway.
Main Methods:
- Quantitative real-time PCR to assess MAPKAPK5-AS1 expression levels in NSCLC tissues and cells.
- Cell proliferation, migration, and apoptosis assays to evaluate the functional impact of MAPKAPK5-AS1.
- Western blotting and dual-luciferase reporter assays to confirm the interaction between MAPKAPK5-AS1, miR-515-5p, and CAB39.
- Rescue experiments to validate the role of the MAPKAPK5-AS1/miR-515-5p/CAB39 axis in NSCLC progression.
Main Results:
- MAPKAPK5-AS1 was significantly upregulated in NSCLC cells.
- Downregulation of MAPKAPK5-AS1 inhibited NSCLC cell proliferation and migration while promoting apoptosis.
- MAPKAPK5-AS1 acted as a molecular sponge for miR-515-5p, leading to increased calcium-binding protein 39 (CAB39) expression.
- Inhibition of miR-515-5p or overexpression of CAB39 could reverse the suppressive effects of MAPKAPK5-AS1 silencing on NSCLC progression.
Conclusions:
- MAPKAPK5-AS1 facilitates NSCLC progression by upregulating CAB39 expression through sequestering miR-515-5p.
- The MAPKAPK5-AS1/miR-515-5p/CAB39 signaling pathway represents a potential therapeutic target for NSCLC treatment.
- MAPKAPK5-AS1 may serve as a promising diagnostic and prognostic biomarker for NSCLC.
Related Concept Videos
Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Cancer Cell Migration through Invadopodia
2.4K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.4K
Mitogens and the Cell Cycle
6.6K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.6K
Metastasis
5.6K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
5.6K
MAPK Signaling Cascades
5.8K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.8K
Role of Myosin in Cell Migration
2.4K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
2.4K


