Detoxification and removal of arsenite by Pseudomonas sp. SMS11: Oxidation, biosorption and bioaccumulation

Arsenite [As(III)] oxidizing bacteria have been widely studied for their detoxification ability through transforming As(III) into arsenate [As(V)]. However, few was focused on removal capacity of arsenic (As). In the current study, As(III) oxidation accompanied with removal of total As was observed in Pseudomonas sp. SMS11. The biosorption (unbinding and surface binding) and bioaccumulation (intracellular uptake) of As by the cells were investigated. Biosorption isotherm was defined adequately by Langmuir and Freundlich models. Biosorption kinetics was recommended by pseudo second-order model. For comparison, the bacteria were inoculated in pure water or culture media amended with different concentrations of As(III) to evaluate the remediation capacity without or with bacterial growth. After removing unbound As, surface bound and intracellular As were sequentially separated using EDTA elution and acidic extraction from bacterial cells. Without bacterial growth, oxidation of As(III) was retarded and the maximum values of surface bound and intracellular As were 4.8 and 10.5 mg/g, respectively. Efficient oxidation and high adsorption capacity were observed after bacterial growth. The surface bound and intracellular As achieved up to 555.0 and 2421.5 mg/g, respectively. Strain SMS11 exhibited great accumulation capacity of As in aqueous solutions, indicating potential application in detoxification and removal of As(III) contamination. The results also suggested that bioremediation via bacteria should be based on living cells and bacterial growth rate.