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Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

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Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
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Updated: Aug 8, 2025

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Wavelength sequential selection technique for high-throughput multi-channel phase interrogation surface plasmon

Wei Sang1, Songfeng Huang1, Jiajie Chen1

  • 1Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, 518060, China.

Talanta
|March 4, 2023
PubMed
Summary

A new multi-channel phase interrogation surface plasmon resonance imaging (mcP-SPRi) platform enhances biosensor detection range and simplifies configuration. This innovation improves high-throughput biomolecule sensing capabilities.

Keywords:
BiosensorsDynamic detection rangePhase interrogationSurface plasmon resonance imaging

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Area of Science:

  • Biomedical Engineering
  • Optical Biosensing
  • Surface Plasmon Resonance Technology

Background:

  • Phase interrogation surface plasmon resonance (P-SPR) biosensors offer high sensitivity but suffer from limited dynamic detection range and complex setups.
  • Existing P-SPR sensors face challenges in accurately detecting diverse biomolecules due to signal inconsistencies arising from their narrow dynamic range.

Purpose of the Study:

  • To design and develop a novel multi-channel P-SPR imaging (mcP-SPRi) sensing platform to overcome the limitations of conventional P-SPR sensors.
  • To enhance the dynamic detection range and simplify the device configuration of P-SPR biosensors for improved performance.

Main Methods:

  • A common-path ellipsometry scheme was employed to create the mcP-SPRi sensing platform.
  • A wavelength sequential selection (WSS) technique was developed to optimize sensing wavelengths based on sample refractive indexes (RIs).

Main Results:

  • The mcP-SPRi platform achieved a dynamic detection range of 3.7×10-3 RIU, the largest reported for mcP-SPRi biosensors.
  • The WSS technique effectively eliminated signal inconsistencies across different biomolecule types by selecting optimal wavelengths.
  • Individual SPR phase image acquisition time was reduced to 1 second, enabling high-throughput sensing.

Conclusions:

  • The developed mcP-SPRi sensing platform significantly expands the dynamic detection range and simplifies the configuration of P-SPR biosensors.
  • The WSS technique is crucial for achieving high-throughput and reliable biomolecule detection with mcP-SPRi.
  • This advancement holds promise for more efficient and versatile biosensing applications.