Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Aug 8, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.0K

Microfluidic single-cell scale-down systems: introduction, application, and future challenges.

Sarah Täuber1, Alexander Grünberger2

  • 1Multiscale Bioengineering, Technical Faculty, Bielefeld University, Bielefeld, Germany; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany.

Current Opinion in Biotechnology
|March 5, 2023
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In-situ product recovery in microfluidic bioreactors.

Current opinion in biotechnology·2026
Same author

Establishment of a Single-Cell Minimal Medium: A Case Study for Microfluidic Cultivation of Corynebacterium glutamicum.

Biotechnology and bioengineering·2026
Same author

Microfluidic-Based Whole-Cell Biosensor Systems-Challenges and Future Applications.

Biosensors·2026
Same author

Transfer Learning Approaches in Bioprocess Engineering: Opportunities and Challenges.

Biotechnology and bioengineering·2026
Same author

Windows of operation as qualitative early-stage design tool for microfluidic (single-cell) cultivations.

Current opinion in biotechnology·2025
Same author

Decoding bioprocesses with transcriptomics: current status and future potential.

Biotechnology advances·2025
Same journal

Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design.

Current opinion in biotechnology·2026
Same journal

Medicinal plants fermentation: current knowledge and perspectives.

Current opinion in biotechnology·2026
Same journal

Fermented foods: lessons learned from metagenomics.

Current opinion in biotechnology·2026
Same journal

Microfluidic platforms for the transient transfection of mammalian cells: recent developments and challenges.

Current opinion in biotechnology·2026
Same journal

Harvesting insights from recent advances in yeast genomics for predictable and precision wine fermentation.

Current opinion in biotechnology·2026
Same journal

Minimal enzyme cascades for the aromatic-to-aromatic upgrading of lignin monomers.

Current opinion in biotechnology·2026
See all related articles

Scale-down bioreactors help transfer bioprocesses but average cell behavior. Microfluidic single-cell cultivation (MSCC) offers single-cell insights but lacks bioprocess relevance, requiring further development for scale-down applications.

Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Cellular Biology

Background:

  • Scaling up bioprocesses faces challenges due to concentration gradients, impacting performance.
  • Scale-down bioreactors are crucial for predicting industrial-scale bioprocess success.
  • Current methods often average cellular behavior, missing critical cell-to-cell heterogeneity.

Purpose of the Study:

  • To review advancements in microfluidic single-cell cultivation (MSCC) systems.
  • To evaluate MSCC systems for analyzing cells under dynamic, bioprocess-relevant conditions.
  • To identify technological needs for using MSCC as single-cell scale-down devices.

Main Methods:

  • Critical review of recent scientific literature on MSCC systems.
  • Analysis of MSCC capabilities for dynamic cultivation and environmental condition simulation.

More Related Videos

Development of New Therapeutic Applications Using Microfluidics
08:56

Development of New Therapeutic Applications Using Microfluidics

Published on: October 1, 2007

5.5K
Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
07:05

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

6.1K

Related Experiment Videos

Last Updated: Aug 8, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.0K
Development of New Therapeutic Applications Using Microfluidics
08:56

Development of New Therapeutic Applications Using Microfluidics

Published on: October 1, 2007

5.5K
Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
07:05

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

6.1K
  • Assessment of current MSCC limitations in replicating industrial bioprocess environments.

    • Main Results:

    • MSCC systems provide single-cell resolution, overcoming averaged measurements in traditional scale-down models.
    • Recent MSCC developments enable cultivation under dynamic conditions relevant to bioprocesses.
    • Existing MSCC systems often lack the parameter range and representativeness needed for bioprocess simulation.

    Conclusions:

    • MSCC offers a powerful approach to study cell heterogeneity during bioprocess scale-up.
    • Technological advancements are necessary to enhance MSCC systems for bioprocess-relevant scale-down studies.
    • Bridging the gap requires developing MSCC with more comprehensive and dynamic environmental controls.