Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.9K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.9K
Computed Tomography01:10

Computed Tomography

4.7K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
4.7K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.3K
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

33
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
33
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.9K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Label-free multimodal nonlinear microscopy enabled by an optical parametric generator.

APL photonics·2026
Same author

Label-free correlative morpho-chemical tomography of 3D kidney mesangial cells.

Journal of biomedical optics·2026
Same author

Unified Vibrational and Multiphoton Label-Free Nonlinear Microscopy for Simultaneous Chemical and Structural Imaging.

IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society·2026
Same author

Shape-conformal porous frameworks for full coverage of neural organoids and high-resolution electrophysiology.

Nature biomedical engineering·2026
Same author

Snapshot hyperspectral fundus imaging system using a microlens array.

Biomedical optics express·2026
Same author

Corrections to "Nonlinear Imaging Histopathology: A Pipeline to Correlate Gold-Standard Hematoxylin and Eosin Staining With Modern Nonlinear Microscopy".

IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society·2026
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Aug 8, 2025

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.6K

Tunable image-mapping optical coherence tomography.

Jaeyul Lee1,2, Xiaoxi Du1,2, Jongchan Park1

  • 1Department of Bioengineering, University of California, Los Angeles, 410 Westwood Plaza, Los Angeles, California, 90095, USA.

Biomedical Optics Express
|March 6, 2023
PubMed
Summary
This summary is machine-generated.

We developed tunable image-mapping optical coherence tomography (TIM-OCT) for flexible, high-resolution imaging. This adaptable system optimizes performance for specific applications without moving parts.

More Related Videos

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

11.6K
Application of Optical Coherence Tomography to a Mouse Model of Retinopathy
08:22

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

Published on: January 12, 2022

4.2K

Related Experiment Videos

Last Updated: Aug 8, 2025

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.6K
Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

11.6K
Application of Optical Coherence Tomography to a Mouse Model of Retinopathy
08:22

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

Published on: January 12, 2022

4.2K

Area of Science:

  • Biomedical Optics
  • Optical Imaging
  • Microscopy

Background:

  • Optical coherence tomography (OCT) is a valuable non-invasive imaging technique.
  • Achieving high resolution in all dimensions simultaneously remains a challenge for conventional OCT systems.
  • Existing OCT methods often require mechanical scanning, limiting speed and introducing artifacts.

Purpose of the Study:

  • To introduce a novel tunable image-mapping optical coherence tomography (TIM-OCT) system.
  • To demonstrate the system's ability to optimize imaging performance for specific applications.
  • To showcase the integration of TIM-OCT with computational adaptive optics.

Main Methods:

  • Utilized a programmable phase-only spatial light modulator within a low-coherence full-field spectral-domain interferometer.
  • Implemented single-shot acquisition for high lateral or axial resolution.
  • Employed multiple-shot acquisition for high resolution across all dimensions.
  • Integrated computational adaptive optics for aberration correction.

Main Results:

  • The TIM-OCT system achieved tunable, optimized imaging performance.
  • Demonstrated the capability for high lateral or axial resolution in a single snapshot.
  • Showcased high resolution in all dimensions via multiple-shot acquisition.
  • Successfully corrected sample-induced optical aberrations using integrated adaptive optics.

Conclusions:

  • TIM-OCT offers a flexible and powerful platform for advanced optical imaging.
  • The system's adaptability and aberration correction capabilities enhance its utility for diverse applications.
  • This technology holds significant potential for biological sample analysis and other scientific fields.