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Analysis of Fat Graft Survival and Platelet-Rich Plasma Effects: The Transcriptomic Differences
Ecem E Yeğin1, Mehmet E Yeğin2, Buket Kosova3
1Bioinformatics, Ege University, Izmir, TUR.
Introduction:
Fat graft survival has been studied numerously but has not gone beyond hypothetical solutions. The molecular changes in survival of standard fat grafts and enhanced survival by platelet-rich plasma (PRP) are compared in this study to reveal the etiology that causes the loss of fat grafts after transplantation.
Materials And Methods:
A New Zealand rabbit's inguinal fat pads were excised and divided into three groups: Sham, Control (C), and PRP. Each weighing 1 g, C and PRP fat were placed into the bilateral parascapular area of the rabbit. After 30 days, the remaining fat grafts were harvested and weighed (C = 0.7 g, PRP = 0.9 g). All three specimens were put into transcriptome analysis. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes Analysis were done to compare the genetic pathways between the specimens.
Results:
Transcriptome analysis showed similar differential expressions in Sham vs. PRP and Sham vs. C comparisons, indicating the dominance of the cellular immune response in both C and PRP specimens. C and PRP comparison resulted in inhibited migration and inflammation pathways in PRP.
Conclusion:
Fat graft survival is more related to immune responses than any other physiological process. PRP enhances survival by attenuating cellular immune reactions.
