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A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain
Yuting Shuai1, Yi Ju1, Yuanhang Li1
1State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST), Shanghai, 200237, China.
Synthetic and Systems Biotechnology
|March 6, 2023
Summary
A new Sulfur DNA mediated nucleic acid sensing platform (SENSOR) offers rapid, sensitive detection of nucleic acids, including single nucleotide polymorphisms, within 10 minutes.
Area of Science:
- Molecular Biology
- Biotechnology
- Diagnostics
Background:
- Nucleic acid detection is crucial for disease diagnosis and control.
- Existing methods face limitations in speed, simplicity, precision, and cost.
Purpose of the Study:
- To develop a novel, rapid nucleic acid detection method.
- To overcome the trade-offs of current nucleic acid detection techniques.
Main Methods:
- Developed SENSOR (Sulfur DNA mediated nucleic acid sensing platform) using phosphorothioate (PT)-DNA and a sulfur binding domain (SBD).
- Utilized PT-DNA oligos and SBD as a targeting module linked to a split luciferase reporter.
- Achieved detection within 10 minutes.
Main Results:
- Demonstrated attomolar sensitivity for nucleic acid detection, including synthesized nucleic acids and a COVID-19 pseudovirus.
- Successfully discriminated single nucleotide polymorphisms (SNPs).
- The SENSOR platform achieved high sensitivity with an amplification procedure.
Conclusions:
- SENSOR is a novel and promising technique for rapid nucleic acid detection.
- The platform offers high sensitivity and specificity.
- SENSOR has potential applications in diverse diagnostic and disease control scenarios.
