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Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
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Antiproliferative activity of antimicrobial peptides and bioactive compounds from the mangrove Glutamicibacter

Yalpi Karthik1, Manjula Ishwara Kalyani1, Srinivasa Krishnappa2

  • 1Department of Studies and Research in Microbiology, Mangalore University, Mangalore, Karnataka, India.

Frontiers in Microbiology
|March 6, 2023
PubMed

Insights

This study isolated *Glutamicibacter mysorens* from Indian mangrove soil, revealing small bioactive compounds with both antimicrobial and anticancer properties, offering potential for new therapeutics.

Area of Science:

  • Microbiology
  • Biochemistry
  • Pharmacology

Background:

  • The *Glutamicibacter* genus is recognized for producing valuable antibiotics and enzymes.
  • These microbial products play a role in managing chronic human diseases.

Purpose of the Study:

  • To isolate and characterize *Glutamicibacter mysorens* from Indian mangrove soil.
  • To identify and evaluate the pharmacological potential of its bioactive compounds.

Main Methods:

  • Isolation and cultivation of *G. mysorens* on starch casein agar.
  • Micromorphological analysis using Field Emission Scanning Electron Microscopy (FESEM).
  • Characterization of intracellular extracts via Gas Chromatography-Mass Spectrometry (GCMS) and Liquid Chromatography-Mass Spectrometry (LC-MS).
  • Anticancer activity assessment on prostate cancer cell lines after Sephadex G-10 purification.

Main Results:

  • *G. mysorens* strain MW647910.1 exhibited a unique spirally coiled spore chain morphology.
  • GCMS and LC-MS identified low molecular weight (<1 kDa) bioactive compounds, including Kinetin-9-ribose and Embinin.
  • Purified protein fractions demonstrated significant anticancer activity against prostate cancer cells.

Conclusions:

  • *G. mysorens* produces small molecular weight compounds with dual antimicrobial and anticancer activities.
  • These microbial-derived compounds show promise as future therapeutic agents.
  • The study highlights the potential of microbial resources for novel drug discovery.
Keywords:
FESEMGlutamicibacter mysorensanticancerchromatographymangrove soilmicrobial peptides

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