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Updated: Aug 8, 2025

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
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Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Published on: February 21, 2019

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Analyzing the interactome of human CK2β in prostate carcinoma cells reveals HSP70-1 and Rho guanin nucleotide

Anna Nickelsen1, Claudia Götz2, Florian Lenz1

  • 1Institute of Pharmaceutical and Medicinal Chemistry University of Münster Münster Germany.

FASEB Bioadvances
|March 6, 2023
PubMed

Insights

Researchers identified new proteins interacting with CK2β, a key part of protein kinase CK2. This reveals CK2β

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • The function of CK2β, the non-catalytic subunit of protein kinase CK2, remains largely unknown.
  • Understanding CK2β's interactions is crucial for elucidating its role in cellular processes.

Purpose of the Study:

  • To identify novel interaction partners of human CK2β.
  • To characterize the interaction between CK2β and Heat Shock Protein 70-1 (HSP70-1).
  • To investigate the potential involvement of CK2β in the Rho-GTPase signaling pathway.

Main Methods:

  • Photo-crosslinking and mass spectrometry were employed to identify CK2β interacting proteins from prostate cancer cell lysates.
  • Microscale thermophoresis was used to quantify the binding affinity (KD) between CK2β and HSP70-1.
  • Co-immunoprecipitation experiments were performed in multiple cancer cell lines to validate in vivo interactions.

Main Results:

  • 38 new interaction partners of CK2β were identified, including HSP70-1 with high abundance.
  • The dissociation constant (KD) for the CK2β-HSP70-1 interaction was determined to be 0.57 μM, with evidence for an interaction independent of CK2 activity.
  • Rho guanine nucleotide exchange factor 12 was identified as another CK2β partner, suggesting a role in the Rho-GTPase pathway and cytoskeleton organization.

Conclusions:

  • This study significantly expands the known interactome of CK2β, identifying numerous novel partners.
  • The characterization of the CK2β-HSP70-1 interaction provides quantitative data and suggests a functional link independent of kinase activity.
  • The identification of Rho guanine nucleotide exchange factor 12 as a CK2β partner implicates CK2β in cytoskeleton regulation via the Rho-GTPase pathway.
Keywords:
HSP70‐1Rho guanin nucleotide exchange factor 12photo‐crosslinking mass spectrometryprostate cancerprotein kinase CK2

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