Jove
Visualize
Contact Us

Related Concept Videos

Folliculogenesis01:20

Folliculogenesis

928
Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
928
The Nucleolus02:55

The Nucleolus

8.9K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a Machine Learning Model for Predicting Treatment-Related Amenorrhea in Young Women with Breast Cancer.

Bioengineering (Basel, Switzerland)·2025
Same author

Proteomic profiling reveals the molecular signatures of chemotherapy-induced human ovarian damage.

Human reproduction (Oxford, England)·2025
Same author

Corrigendum to "Hormonal factors predictive of fertility in patients with breast cancer interrupting adjuvant endocrine therapy to attempt pregnancy in POSITIVE trial" [The Breast 83 (2025) 104547].

Breast (Edinburgh, Scotland)·2025
Same author

Hormonal factors predictive of fertility in patients with breast cancer interrupting adjuvant endocrine therapy to attempt pregnancy in POSITIVE trial.

Breast (Edinburgh, Scotland)·2025
Same author

Carboplatin and paclitaxel induced-gonadotoxicity on the ovarian reserve of young breast cancer patients with BRCA1 mutation.

Human reproduction (Oxford, England)·2025
Same author

Breastfeeding after breast cancer in young BRCA carriers.

Journal of the National Cancer Institute·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Aug 8, 2025

Gene Expression Analyses in Human Follicles
09:09

Gene Expression Analyses in Human Follicles

Published on: February 17, 2023

1.2K

Gene Expression Analyses in Human Follicles.

Melody Devos1, Joana Dias Nunes1, Isabelle Demeestere2

  • 1Research Laboratory on Human Reproduction, Université libre de Bruxelles (ULB).

Journal of Visualized Experiments : Jove
|March 6, 2023
PubMed
Summary
This summary is machine-generated.

This study details a refined protocol for isolating human ovarian follicles, crucial for studying gene expression during folliculogenesis. The method ensures adequate RNA yield for real-time quantitative polymerase chain reaction (RT-qPCR) analysis.

More Related Videos

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration
09:11

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration

Published on: September 27, 2022

3.6K
Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles
06:36

Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles

Published on: November 30, 2022

1.3K

Related Experiment Videos

Last Updated: Aug 8, 2025

Gene Expression Analyses in Human Follicles
09:09

Gene Expression Analyses in Human Follicles

Published on: February 17, 2023

1.2K
Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration
09:11

Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration

Published on: September 27, 2022

3.6K
Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles
06:36

Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles

Published on: November 30, 2022

1.3K

Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Ovarian folliculogenesis involves complex molecular mechanisms.
  • Accurate assessment of gene expression in human follicles requires their isolation.
  • Previous methods for follicle isolation were suboptimal for downstream molecular analyses.

Purpose of the Study:

  • To present an adapted protocol for the isolation of human ovarian follicles.
  • To enable molecular studies, including gene expression analysis, on isolated follicles.
  • To optimize the yield and quality of RNA from isolated follicles for RT-qPCR.

Main Methods:

  • Manual processing of ovarian cortical tissue using a tissue slicer and chopper.
  • Enzymatic digestion with collagenase and DNase, combined with mechanical pipetting.
  • Microscopic isolation of follicles using a calibrated microcapillary pipette and microdissection.
  • Controlled digestion time (max 90 min) and stopping reaction with a blocking solution.

Main Results:

  • Successful isolation of human ovarian follicles (oocyte and granulosa cells).
  • A minimum of 20 isolated follicles (under 75 µm) yields adequate total RNA (mean 5 ng/µL).
  • Isolated RNA is suitable for cDNA synthesis and subsequent RT-qPCR analysis.

Conclusions:

  • The described protocol effectively isolates human ovarian follicles for molecular studies.
  • This method provides sufficient RNA for gene expression analysis via RT-qPCR.
  • The protocol is essential for advancing research into the molecular aspects of human folliculogenesis.