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Aptamer-based Upconversion Fluorescence Sensor for Doxorubicin Detection.

Jingwen Mo1, Shichang Wang2, Jiaying Zeng3

  • 1Jiangsu Key Laboratory for Design & Manufacture of Micro/Nano Biomedical Instruments, Engineering Research Center of New Light Sources Technology & Equipment-Ministry of Education, and School of Mechanical Engineering, Southeast University, Nanjing, 210096, People's Republic of China. jwmo@seu.edu.cn.

Journal of Fluorescence
|March 6, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a novel aptamer-based sensor for detecting doxorubicin (DOX), a crucial chemotherapy drug. The sensor utilizes upconversion fluorescence for precise and sensitive monitoring of doxorubicin levels in biological samples.

Keywords:
808 nm-excited upconversion nanoparticlesAptamersDoxorubicinFluorescence sensors

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Doxorubicin (DOX) is a vital chemotherapy agent for various cancers.
  • Accurate monitoring of DOX concentrations in biological fluids is essential for effective cancer treatment.
  • Existing detection methods may lack specificity or sensitivity for therapeutic drug monitoring.

Purpose of the Study:

  • To develop a highly specific and sensitive aptamer-functionalized upconversion fluorescence sensor for doxorubicin detection.
  • To investigate the sensor's performance in detecting doxorubicin in complex biological matrices like urine.
  • To establish a reliable method for therapeutic drug monitoring of doxorubicin.

Main Methods:

  • Fabrication of core-shell upconversion nanoparticles functionalized with doxorubicin-specific aptamers.
  • Utilizing fluorescence resonance energy transfer (FRET) between upconversion nanoparticles (donor) and doxorubicin (acceptor) for signal generation.
  • Characterization of the sensor's response to varying doxorubicin concentrations and its specificity against interfering substances.

Main Results:

  • The aptasensor demonstrated a linear response to doxorubicin concentrations from 0.5 μM to 55 μM.
  • A low detection limit of 0.5 μM was achieved for doxorubicin.
  • The sensor exhibited high specificity and anti-interference capabilities against common antibiotics, ions, and biomolecules.
  • Successful application in detecting doxorubicin in urine samples with nearly 100% spike recovery.

Conclusions:

  • The developed aptamer-functionalized upconversion fluorescence sensor provides a sensitive and specific method for doxorubicin detection.
  • This sensor shows significant potential for real-time therapeutic drug monitoring of doxorubicin in clinical settings.
  • The aptasensor's performance in urine samples highlights its applicability for practical biological fluid analysis.