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Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping
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Distributed optical fiber biosensor based on optical frequency domain reflectometry.

Peidong Hua1, Zhenyang Ding1, Kun Liu1

  • 1School of Precision Instruments and Opto-Electronics Engineering, Tianjin University, Tianjin, 300072, China; Tianjin Optical Fiber Sensing Engineering Center, Institute of Optical Fiber Sensing of Tianjin University, Tianjin, 300072, China; Key Laboratory of Opto-electronics Information Technology (Tianjin University), Ministry of Education, Tianjin, 300072, China.

Biosensors & Bioelectronics
|March 6, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a novel distributed optical fiber biosensor using optical frequency domain reflectometry (OFDR) for real-time biochemical substance detection. It achieves micron-level localization of analytes, advancing beyond single-point measurements for applications like cancer detection.

Keywords:
BiosensorDistributed optical fiber biosensorDistributed optical fiber sensingOptical frequency domain reflectometryTapered fiber

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Area of Science:

  • Biomedical Engineering
  • Optical Sensing
  • Biochemistry

Background:

  • Accurate spatial distribution of biochemical substances is crucial for cell analysis and cancer detection.
  • Existing optical fiber biosensors offer label-free, fast measurements but are limited to single-point detection.
  • There is a need for distributed biosensing technologies to map biochemical substance concentrations spatially.

Purpose of the Study:

  • To develop and demonstrate the first distributed optical fiber biosensor using optical frequency domain reflectometry (OFDR).
  • To enable label-free, real-time, and spatially resolved detection of biochemical substances.
  • To advance biosensing capabilities from single-point measurements to distributed mapping.

Main Methods:

  • Fabrication of a tapered optical fiber (6 μm waist diameter, 140 mm length) to enhance the evanescent field.
  • Immobilization of a human IgG layer on the tapered region using polydopamine (PDA) for anti-human IgG detection.
  • Utilizing OFDR to measure shifts in local Rayleigh backscattering spectra (RBS) due to refractive index changes.

Main Results:

  • Demonstrated linearity between anti-human IgG concentration (0-14 ng/ml) and RBS shift over a 50 mm sensing range.
  • Achieved a concentration measurement limit of 2 ng/ml for anti-human IgG.
  • Obtained an ultra-high spatial resolution of 680 μm for locating anti-human IgG concentration changes.

Conclusions:

  • The proposed distributed biosensor based on OFDR successfully achieves spatially resolved biochemical substance detection.
  • This technology has the potential to localize substances like cancer cells at the micron level.
  • It represents a significant advancement from single-point biosensors to distributed sensing systems.