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MicroRNA-324-3p inhibits osteosarcoma progression by suppressing PGAM1-mediated aerobic glycolysis

Yiping Weng1,2, Weihao Duan2, Xuecheng Yu2

  • 1State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.

Cancer Science
|March 7, 2023
PubMed

Insights

MicroRNA-324-3p suppresses osteosarcoma progression by inhibiting the Warburg effect. This microRNA targets PGAM1, offering a potential therapeutic strategy for bone cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma (OS) is a primary bone cancer with limited treatment options.
  • MicroRNA (miR)-324-3p's role in OS progression and its mechanisms are largely unknown.
  • The Warburg effect, altered glucose metabolism, is implicated in cancer development.

Purpose of the Study:

  • To investigate the biological roles and mechanisms of miR-324-3p in osteosarcoma progression.
  • To explore the relationship between miR-324-3p, PGAM1, and the Warburg effect in OS.
  • To evaluate the therapeutic potential of targeting the miR-324-3p/PGAM1 axis.

Main Methods:

  • Assessed miR-324-3p expression in OS cell lines and tissues.
  • Overexpressed miR-324-3p to evaluate its functional impact on OS progression and the Warburg effect.
  • Identified PGAM1 as a direct target of miR-324-3p using 3'-UTR analysis.
  • Correlated PGAM1 expression with OS progression, aerobic glycolysis, and patient survival.

Main Results:

  • miR-324-3p expression was significantly reduced in OS.
  • Overexpression of miR-324-3p inhibited OS cell proliferation and migration, and reduced aerobic glycolysis.
  • miR-324-3p directly targeted and downregulated PGAM1 expression.
  • High PGAM1 expression promoted OS progression and aerobic glycolysis, correlating with poor patient survival.
  • PGAM1 overexpression partially rescued the tumor-suppressive effects of miR-324-3p.

Conclusions:

  • The miR-324-3p/PGAM1 axis is a critical regulator of osteosarcoma progression via modulation of the Warburg effect.
  • miR-324-3p acts as a tumor suppressor by inhibiting aerobic glycolysis in OS.
  • Targeting the miR-324-3p/PGAM1 pathway presents a promising molecular strategy for osteosarcoma treatment.
Keywords:
PGAM1Warburg effectmiR-324-3posteosarcomatherapeutic target

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