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MiR-146b-5p/SEMA3G regulates epithelial-mesenchymal transition in clear cell renal cell carcinoma

Mengxi Tang1, Tao Xiong2

  • 1Urinary Surgery, The People's Hospital of Rongchang District, Chongqing, 402460, China.

Cell Division
|March 7, 2023
PubMed
Abstract

Insights

MicroRNA-146b-5p promotes clear cell renal cell carcinoma (ccRCC) growth by inhibiting SEMA3G. This axis impacts cell invasion, migration, and epithelial-mesenchymal transition (EMT), offering potential therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Clear cell renal cell carcinoma (ccRCC) is a significant health concern.
  • The role of microRNAs (miRNAs) in cancer progression is increasingly recognized.
  • Understanding specific miRNA-mRNA interactions is crucial for identifying therapeutic targets.

Purpose of the Study:

  • To elucidate the functional role of the miR-146b-5p/SEMA3G axis in ccRCC.
  • To investigate the molecular mechanisms by which miR-146b-5p influences ccRCC progression.
  • To explore the potential of this axis as a therapeutic target for ccRCC.

Main Methods:

  • Utilized TCGA database for ccRCC data analysis, including survival analysis.
  • Performed miRNA target prediction and intersected with differential mRNAs.
  • Assessed protein expression (SEMA3G, EMT markers, signaling pathways) via qRT-PCR and Western blot.
  • Validated miRNA-mRNA interaction using dual-luciferase assay.
  • Evaluated cell migration, invasion, and morphology changes through Transwell and wound healing assays.

Main Results:

  • miR-146b-5p was significantly overexpressed in ccRCC, while SEMA3G expression was reduced.
  • miR-146b-5p promoted ccRCC cell invasion, migration, and epithelial-mesenchymal transition (EMT).
  • miR-146b-5p directly targeted and inhibited SEMA3G, consequently regulating Notch and TGF-β signaling pathways.

Conclusions:

  • MiR-146b-5p promotes ccRCC cell growth by suppressing SEMA3G expression.
  • The miR-146b-5p/SEMA3G axis regulates key signaling pathways involved in tumor progression.
  • This axis represents a potential therapeutic target and prognostic biomarker for ccRCC.
Keywords:
Clear cell renal cell carcinomaEpithelial-mesenchymal transitionMetastasisSEMA3GmiR-146b-5p

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