Comparison of regional differences in cardiac myocyte dimensions in rats, hamsters, and guinea pigs

Isolated myocytes were prepared from Sprague Dawley rats, golden Syrian hamsters, and Hartley guinea pigs to investigate regional variations in myocyte size. Cell volume (V) was measured with a Coulter Channelyzer, cell length (L) was measured directly, and cross-sectional area (CSA) was calculated from V/L. Compared to values from the left ventricle (LV), right ventricular L was shorter in the rat (P less than .01) and hamster (P less than .05) and longer in the guinea pig (P less than .01). Guinea pig atrial L was shorter (P less than .01) than L in the right ventricle (RV) but did not differ from L in the LV. No significant differences in L existed between endomyocardium, middle myocardium, and epimyocardium of the LV in all three species. In rats and hamsters, myocytes from the RV had smaller V and CSA values (P less than .01) compared to any region of the LV. A transmural gradient of cellular dimensions existed in the LV of the rat, but not in hamster, with V and CSA of endomyocardium being largest and epimyocardium smallest (P less than .01). Endomyocardial V and CSA were larger (P less than .01) than all other regions in the hamster, but the difference was not significant compared to epimyocardial V. In the guinea pig, no significant differences in V existed between RV and LV or between the three LV regions. No pattern of regional differences was seen between ventricular CSA values in the guinea pig. Guinea pig atrial V and CSA values were smaller (P less than .01) than those for ventricular myocytes.(ABSTRACT TRUNCATED AT 250 WORDS)