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Related Experiment Video

Updated: Aug 7, 2025

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

DNA Modifications Enabling Proximity Biotinylation.

Brandon Wilbanks¹, Keenan Pearson¹, Shane R Byrne²

¹Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine and Science, Rochester, Minnesota 55905, United States.

Bioconjugate Chemistry

|March 8, 2023

Summary

This summary is machine-generated.

Novel methods enable proximity biotinylation of exogenous oligodeoxyribonucleotides using enzymatic tools. This advances cellular mapping and offers potential for selecting nucleic acids that can enter living cells.

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Area of Science:

Biochemistry
Molecular Biology
Chemical Biology

Background:

Peroxidase and biotin ligase-mediated signal amplification allow high-resolution subcellular mapping of RNA and protein interactions.
Current methods are restricted to RNA and proteins due to specific biotinylation requirements.

Purpose of the Study:

To develop novel proximity biotinylation methods for exogenous oligodeoxyribonucleotides.
To expand the application of biotinylation technologies to nucleic acids.

Main Methods:

Utilized well-established enzymatic tools for proximity biotinylation.
Developed conjugation chemistries to modify deoxyribonucleotides with antennae.
Investigated reactions with phenoxy radicals and biotinoyl-5 -adenylate.

Main Results:

Successfully demonstrated proximity biotinylation of exogenous oligodeoxyribonucleotides.
Reported novel chemical details of a tryptophan-phenoxy radical adduct.
Established efficient conjugation chemistries for deoxyribonucleotide modification.

Conclusions:

These advancements enable proximity biotinylation of exogenous nucleic acids.
The developed methods have potential applications in selecting nucleic acids for cellular entry.
Expanded the scope of biotinylation-based mapping technologies to include exogenous DNA.