Regenerative marine waste towards CaCO₃ nanoformulation for Alzheimer's therapy

Alzheimer's disorder (AD) is associated with behavioural and cognitive destruction with due respect to the neurological degeneration. Conventional therapeutic approach for treatment of AD using neuroprotective drugs suffered certain limitations such as poor solubility, insufficient bioavailability, adverse side effects at higher dose and ineffective permeability on blood brain barrier (BBB). Development of nanomaterial based drug delivery system helped to overcome these barriers. Hence the present work focused on encapsulating neuroprotective drug citronellyl acetate within CaCO₃ nanoparticles to develop neuroprotective CaCO₃ nanoformulation (CA@CaCO₃ NFs). CaCO₃ was derived from marine conch shell waste, while the neuroprotective drug citronellyl acetate was scrutinized by in-silico high throughput screening. In-vitro findings revealed that CA@CaCO₃ nanoformulation exhibited enhanced free radical scavenging activity of 92% (IC₅₀ value - 29.27 ± 2.6 μg/ml), AChE inhibition of 95% (IC₅₀ value - 25.6292 ± 1.5 μg/ml) at its maximum dose (100 μg/ml). CA@CaCO₃ NFs attenuated the aggregation of β-amyloid peptide (Aβ) and also disaggregated the preformed mature plaques the major risk factor for AD. Overall, the present study reveals that CaCO₃ nanoformulations exhibits potent neuroprotective potential when compared to the CaCO₃ nanoparticles alone and citronellyl acetate alone due to the sustained drug release and synergistic effect of CaCO₃ nanoparticles and citronellyl acetate depicting the fact that CaCO₃ can act as promising drug delivery system for treatment of neurodegenerative and CNS related disorders.