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Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
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Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders

Published on: December 5, 2016

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Draft genome of the oriental garden lizard (Calotes versicolor)

Qing Wang1,2, Fengping He3, Ru-Yi Huang4

  • 1Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants (Ministry of Education), College of Forestry, Hainan University, Haikou, China.

Frontiers in Genetics
|March 9, 2023
PubMed
Abstract

No abstract available in PubMed .

Keywords:
Calotes versicolordraft genomeoxford nanopore technologies (ONT)phylogenomic analysisreptile

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Lampbrush Chromosomes01:51

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In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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