Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enlargement of the Plasma Membrane01:22

Enlargement of the Plasma Membrane

2.0K
Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...
2.0K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

2.0K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oncogenic Ras drives EED degradation and PRC2 dysfunction to promote aggressive squamous cell carcinoma.

Nature communications·2026
Same author

SIMCROST: A Simulator for Understanding the Spatial Regulation in Cross-Membrane Signal Transduction.

Chembiochem : a European journal of chemical biology·2026
Same author

MISSTE: a multiscale integrative spatial simulator for understanding the mechanisms underlying tissue ecosystems.

bioRxiv : the preprint server for biology·2026
Same author

Effect of early exercise on functional recovery and surgical outcomes after post-mastectomy breast reconstruction: study protocol for a randomized clinical trial.

BMC cancer·2026
Same author

IDBSpred: An intrinsically disordered binding site predictor using machine learning and protein language model.

bioRxiv : the preprint server for biology·2026
Same author

Multiscale conformational sampling of multidomain fusion proteins by a physics informed diffusion model.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Aug 7, 2025

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
10:26

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

12.3K

Fine-tuning spatial-temporal dynamics and surface receptor expression support plasma cell-intrinsic longevity.

Zhixin Jing, Phillip Galbo, Luis Ovando

    Biorxiv : the Preprint Server for Biology
    |March 9, 2023
    PubMed
    Summary

    Long-lived plasma cells (LLPCs) are crucial for durable immunity. This study reveals LLPCs are sessile, cluster-forming cells with unique molecular markers, and CXCR4 is vital for their bone marrow survival and antibody production.

    More Related Videos

    Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
    09:24

    Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

    Published on: January 30, 2020

    8.1K
    An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics
    09:25

    An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics

    Published on: December 24, 2015

    9.0K

    Related Experiment Videos

    Last Updated: Aug 7, 2025

    In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
    10:26

    In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

    Published on: January 20, 2019

    12.3K
    Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
    09:24

    Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

    Published on: January 30, 2020

    8.1K
    An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics
    09:25

    An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics

    Published on: December 24, 2015

    9.0K

    Area of Science:

    • Immunology
    • Cell Biology
    • Systems Biology

    Background:

    • Durable serological memory relies on long-lived plasma cells (LLPCs).
    • Factors governing LLPC generation and survival are not fully understood.

    Approach:

    • Utilized intra-vital two-photon imaging to observe LLPC behavior in vivo.
    • Employed deep bulk RNA sequencing and flow cytometry for comprehensive molecular profiling.
    • Investigated the role of CXCR4 by conditional deletion in plasma cells (PCs).

    Key Points:

    • LLPCs are sessile and form clusters dependent on the survival factor April.
    • LLPCs exhibit a distinct transcriptome and proteome, expressing unique cell surface markers like CD93, CD81, CXCR4, CD326, CD44, and CD48.
    • CXCR4 is essential for LLPC homing to the bone marrow and maintaining antibody titers; its deletion causes rapid mobilization and reduced PC survival.

    Conclusions:

    • LLPC specification appears non-random, with a less diverse B cell receptor repertoire in naive mice.
    • Aging leads to LLPC enrichment in the bone marrow, potentially limiting new PC entry.
    • Understanding LLPC biology is critical for developing effective vaccines and immunotherapies.