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A Multimodal SIMS/MALDI Mass Spectrometry Imaging Source with Secondary Electron Imaging Capabilities for Use with

Kasper Krijnen1, Joel D Keelor1, Sebastian Böhm2

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Summary

This study enhances multimodal mass spectrometry imaging (MSI) by integrating secondary ion mass spectrometry (SIMS) and secondary electron (SE) imaging onto a single instrument, improving efficiency and reducing sample handling for biological research.

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Area of Science:

  • Analytical Chemistry
  • Biomedical Research
  • Surface Science

Background:

  • Mass spectrometry imaging (MSI) is crucial for chemical analysis in biological and biomedical research.
  • Multimodal imaging offers comprehensive sample analysis but often requires multiple instruments, causing registration issues and sample degradation.
  • Current multimodal MSI workflows face challenges with image registration and sample integrity due to instrument transfers.

Purpose of the Study:

  • To enhance multimodal mass spectrometry imaging (MSI) efficiency and explore complementary imaging modes.
  • To develop a single instrument capable of performing multiple MSI techniques simultaneously.
  • To address limitations of current multimodal MSI by minimizing sample transfer and registration complexities.

Main Methods:

  • Modification of a prototype Bruker timsTOF fleX instrument.
  • Integration of secondary ion mass spectrometry (SIMS) and secondary electron (SE) imaging capabilities.
  • Preservation of existing matrix-assisted laser desorption/ionization (MALDI) imaging functionality.
  • Characterization and comparison of SIMS, SE, and MALDI imaging performance.

Main Results:

  • Successful integration of SIMS and SE imaging onto a single MSI instrument alongside MALDI.
  • Acquisition of multimodal images with minimal registration requirements.
  • Demonstration of multimodal imaging without sample transfer between acquisition modes.
  • Characterization of individual imaging mode performance and comparison with commercial systems.

Conclusions:

  • The modified timsTOF fleX enables efficient, integrated multimodal MSI.
  • This approach overcomes key challenges in sample handling and image registration for complex biological samples.
  • The instrument provides a versatile platform for advanced chemical imaging in research settings.