Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Systemic Biomarkers of Treatment Response to Methotrexate in People with Painful Knee Osteoarthritis: a Biological Substudy of the PROMOTE Randomised Controlled Clinical Trial.

Osteoarthritis and cartilage·2026
Same author

New hope for neurotrophin targeting in osteoarthritis pain?

Lancet (London, England)·2026
Same author

T Cells in Osteoarthritis: Drivers, Repairers, or Bystanders in Osteoarthritis?

Arthritis & rheumatology (Hoboken, N.J.)·2026
Same author

Click-Cyclized Cell Penetrating Peptides Containing Hydrophobic Proline Derivatives for Efficient Intracellular Delivery.

Angewandte Chemie (International ed. in English)·2025
Same author

Current and future advances in practice: practical management of hand osteoarthritis.

Rheumatology advances in practice·2025
Same author

Development of a translational strategy for using TIMP-3 to inhibit aggrecanase activity in osteoarthritis.

Osteoarthritis and cartilage·2025

Related Experiment Video

Updated: Aug 7, 2025

Detection of Protease Activity by Fluorescent Peptide Zymography
09:56

Detection of Protease Activity by Fluorescent Peptide Zymography

Published on: January 20, 2019

12.8K

Development of Selective ADAMTS-5 Peptide Substrates to Monitor Proteinase Activity.

Milan M Fowkes1, Linda Troeberg2, Paul E Brennan3

  • 1Centre for OA Pathogenesis Versus Arthritis, Kennedy Institute of Rheumatology, University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7FY, United Kingdom.

Journal of Medicinal Chemistry
|March 9, 2023
PubMed
Summary
This summary is machine-generated.

New Förster resonance energy transfer (FRET) peptide substrates offer sensitive detection of a disintegrin and metalloproteinase with thrombospondin type I motifs-5 (ADAMTS-5) activity, crucial for osteoarthritis diagnosis and therapy evaluation.

More Related Videos

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
19:23

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Published on: January 16, 2019

9.3K
Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
10:50

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

16.4K

Related Experiment Videos

Last Updated: Aug 7, 2025

Detection of Protease Activity by Fluorescent Peptide Zymography
09:56

Detection of Protease Activity by Fluorescent Peptide Zymography

Published on: January 20, 2019

12.8K
Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
19:23

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Published on: January 16, 2019

9.3K
Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
10:50

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

16.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Osteoarthritis (OA) involves articular cartilage degradation driven by proteinases like ADAMTS-5.
  • Sensitive detection of proteinase activity is vital for OA diagnosis and treatment monitoring.
  • Existing Förster resonance energy transfer (FRET) probes for ADAMTS-5 lack selectivity and sensitivity.

Purpose of the Study:

  • To develop novel, highly selective, and sensitive FRET peptide substrates for detecting ADAMTS-5 activity.
  • To improve upon existing FRET probes for ADAMTS-5 detection.

Main Methods:

  • Utilized in silico docking and combinatorial chemistry to design and synthesize new FRET peptide substrates.
  • Evaluated substrate cleavage rates, catalytic efficiencies, and selectivity against related proteinases.
  • Assessed the ability of lead substrates to detect low concentrations of ADAMTS-5.

Main Results:

  • Developed lead FRET substrates (3 and 26) with significantly higher cleavage rates and catalytic efficiencies compared to current standards.
  • Demonstrated high selectivity for ADAMTS-5 over ADAMTS-4, MMP-2, and MMP-9.
  • Achieved sensitive detection of ADAMTS-5 at low nanomolar concentrations.

Conclusions:

  • The newly developed FRET substrates represent a significant advancement in detecting ADAMTS-5 activity.
  • These substrates offer improved sensitivity and selectivity, enabling better OA diagnosis and therapeutic evaluation.
  • This work provides a valuable tool for studying OA pathogenesis and developing targeted treatments.