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Development of a 3D Graphene Electrode Dielectrophoretic Device
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Graphene-based microfluidic perforated microelectrode arrays for retinal electrophysiological studies.

Alberto Esteban-Linares1, Xiaosi Zhang2, Hannah H Lee3

  • 1Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, 37235, USA. deyu.li@vanderbilt.edu.

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|March 9, 2023
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Summary

New microfluidic perforated microelectrode arrays (μpMEAs) enable high-resolution imaging and local chemical stimulation for retinal electrophysiology. This innovation advances the study of retinal circuitry and function.

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Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Ophthalmology

Background:

  • Perforated microelectrode arrays (pMEAs) are vital for ex vivo retinal electrophysiology, improving nutrient supply and retinal contact.
  • Limitations of commercial pMEAs include incompatibility with in situ high-resolution optical imaging and lack of microenvironment control.

Purpose of the Study:

  • To develop microfluidic perforated microelectrode arrays (μpMEAs) integrating transparent graphene electrodes and local chemical stimulation capabilities.
  • To enable simultaneous high-resolution optical imaging and electrophysiological recordings for correlating retinal function with anatomy.

Main Methods:

  • Fabrication of microfluidic pMEAs (μpMEAs) with transparent graphene electrodes.
  • Implementation of localized chemical stimulation delivery systems.
  • Performance evaluation using ex vivo retinal ganglion cell recordings stimulated by high K+.

Main Results:

  • Demonstrated μpMEAs' ability to measure electrical responses of retinal ganglion cells to localized stimulation.
  • Achieved high-resolution confocal imaging of retinal tissue on graphene electrodes.
  • Showcased the potential for correlating electrical signals with anatomical structures.

Conclusions:

  • μpMEAs offer enhanced capabilities for retinal electrophysiology by combining imaging and stimulation.
  • This technology facilitates detailed analysis of retinal circuitry and cellular function.
  • μpMEAs are poised to advance research into retinal physiological and pathological mechanisms.