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Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)01:15

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The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Related Experiment Video

Updated: Aug 7, 2025

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
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High-resolution mass spectrometry for glycoproteomics.

Siyue Bo1, Rumeng Zhang1, Lingbo Zhao1

  • 1Center for Clinical Mass Spectrometry, College of Pharmaceutical Sciences, Soochow University, Jiangsu, 215123, China.

Bioanalysis
|March 9, 2023
PubMed
Summary

This study presents a novel glycoproteomics approach. It directly analyzes glycosylation and glycans using combined bottom-up and top-down mass spectrometry for detailed glycoform mapping.

Keywords:
HRMSglycopeptideglycoproteinhigh-resolution mass spectrometrytop-down

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Glycosylation is a crucial post-translational modification impacting protein function.
  • Comprehensive analysis of glycosylation heterogeneity remains challenging.
  • High-resolution mass spectrometry is vital for detailed glycopeptide characterization.

Purpose of the Study:

  • To develop and validate a combined bottom-up and top-down mass spectrometry strategy for glycoproteomics.
  • To enable direct analysis and mapping of glycoforms and their associated glycans.
  • To advance the understanding of glycosylation site occupancy and heterogeneity.

Main Methods:

  • Integration of bottom-up and top-down mass spectrometry workflows.
  • High-resolution mass spectrometry for accurate mass determination and fragmentation analysis.
  • Glycopeptide enrichment and data analysis pipelines for glycoform identification.

Main Results:

  • Successful direct analysis of glycoform-mapped glycosylation.
  • Comprehensive characterization of intact glycans and their site-specific attachment.
  • High sensitivity and specificity in identifying diverse glycoforms.

Conclusions:

  • The combined strategy offers a powerful tool for in-depth glycoproteomics analysis.
  • Direct glycoform mapping provides unprecedented insights into glycosylation.
  • This approach facilitates a deeper understanding of the biological roles of glycosylation.