Design of a highly sensitive and versatile membrane-based immunosensor using a Cu-free click reaction

Area of Science:

• Biomedical Engineering
• Biosensing Technology
• Immunochemistry

Background:

• Developing highly sensitive and specific biosensors is crucial for early disease diagnosis.
• Interleukin-6 (IL-6) is a key biomarker in inflammatory diseases, requiring precise quantification.
• Existing immunosensors often suffer from low sensitivity and non-specific binding, limiting their clinical utility.

Purpose of the Study:

• To develop a highly sensitive immunosensor for rapid detection of interleukin-6 (IL-6).
• To leverage membrane pores as a recognition interface for enhanced sensing performance.
• To improve antibody immobilization and minimize non-specific protein adsorption.

Main Methods:

• Fabrication of an immunosensor utilizing membrane pores as the recognition interface.
• Application of Cu-free click reaction for efficient antibody immobilization.
• Implementation of strategies to inhibit non-specific protein adsorption.

Main Results:

• The developed immunosensor exhibits high sensitivity.
• Efficient antibody immobilization was achieved using Cu-free click chemistry.
• The sensor effectively inhibited non-specific protein adsorption, enhancing signal-to-noise ratio.
• Rapid detection of interleukin-6 was demonstrated in the picogram per milliliter range.

Conclusions:

• The membrane pore-based immunosensor offers a promising platform for sensitive and rapid biomarker detection.
• Cu-free click chemistry provides an effective method for antibody immobilization in biosensor development.
• The sensor's ability to suppress non-specific binding significantly improves detection performance for interleukin-6.