Related Experiment Video
Updated: Aug 7, 2025

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Characterization of Z-DNA by Infrared Spectroscopy
1Henan Key Laboratory of Ion-Beam Bioengineering, School of Physics and Microelectronics, Zhengzhou University, Zhengzhou, China.
Abstract:
Infrared spectrum stems from the matter's absorption of light in the infrared (IR) light region. Generally, this infrared light absorption is due to the transition of vibrational and rotational energy levels of the involved molecule. Since different molecules have different structures and vibration modes, infrared spectroscopy can therefore be widely applied to analyze the chemical compositions and structures of molecules. Here we describe the method of application of infrared spectroscopy in the investigation of Z-DNA in cells, as infrared spectroscopy can distinguish DNA secondary structures sensitively and the band at 930 cm-1 is specifically attributed to the Z-form DNA. Based on the curve fitting, the relative content of Z-DNA in the cells may be evaluated.
More Related Videos
Related Concept Videos
Infrared (IR) Spectroscopy: Overview
Different compounds display unique properties due to their...
IR Frequency Region: Fingerprint Region
IR Spectroscopy: Molecular Vibration Overview
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
IR Spectrum
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0%...
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration
According to Hooke's law, the vibrational frequency is directly proportional to...
IR and UV–Vis Spectroscopy of Aldehydes and Ketones

