Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Real-Time Mid-IR Spectroscopy Inside Reactors with a Modular Endoscopic Probe.

Analytical chemistry·2025

Same author

Water-accelerated photooxidation and degradation of lignin linkages mediated by plasmonic catalysts.

Chemical science·2025

Same author

Deciphering ligand and metal ion dependent intricate folding landscape of Vc2 c-di-GMP riboswitch aptamer.

Nucleic acids research·2025

Same author

Molecular basis of facilitated target search and sequence discrimination of TALE homeodomain transcription factor Meis1.

Nature communications·2024

Same author

A New Perspective on Metabolites and Bioactive Compounds from Fungi.

The American journal of Chinese medicine·2023

Same author

Curcumin Chalcone Derivatives Database (CCDD): a Python framework for natural compound derivatives database.

PeerJ·2023

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 7, 2025

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

NMR Titration Studies in Z-DNA Dynamics.

Seo-Ree Choi¹, Kwang-Im Oh¹, Yeo-Jin Seo¹

¹Department of Chemistry and the Research Institute of Natural Science, Gyeongsang National University, Jinju, South Korea.

Methods in Molecular Biology (Clifton, N.J.)

|March 9, 2023

Summary

This summary is machine-generated.

This study uses Nuclear Magnetic Resonance (NMR) titration to investigate how a protein binds to Z-DNA. The technique monitors protein-DNA interactions and reveals protein-induced DNA conformational changes.

Keywords:

Chemical shift perturbation DNA–protein interaction Dynamics NMR Titration Z-DNA Z-DNA-binding protein

More Related Videos

Magnetic Tweezers for the Measurement of Twist and Torque

Magnetic Tweezers for the Measurement of Twist and Torque

Published on: May 19, 2014

Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example

Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example

Published on: October 26, 2016

Related Experiment Videos

Last Updated: Aug 7, 2025

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

Magnetic Tweezers for the Measurement of Twist and Torque

Magnetic Tweezers for the Measurement of Twist and Torque

Published on: May 19, 2014

Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example

Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example

Published on: October 26, 2016

Area of Science:

Biochemistry
Molecular Biology
Structural Biology

Background:

Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful tool for studying molecular interactions.
Chemical Shift Perturbation (CSP) is an NMR technique used to map protein-DNA binding interfaces.
Understanding protein-DNA interactions is crucial for deciphering biological processes.

Purpose of the Study:

To describe the application of CSP-NMR titration for studying the binding of a protein to Z-DNA.
To investigate protein-induced conformational changes in DNA using NMR.
To analyze DNA binding dynamics and B-Z transition using NMR titration data.

Main Methods:

Utilized ¹⁵N-labeled Z-DNA-binding protein.
Performed unlabeled DNA titration into the protein.
Monitored interactions using 2D heteronuclear single-quantum correlation (HSQC) spectra.
Analyzed NMR titration data with the active B-Z transition model.

Main Results:

Successfully monitored DNA binding to the ¹⁵N-labeled Z-DNA-binding protein via 2D HSQC spectra.
Observed chemical shift perturbations indicative of protein-DNA interactions.
Obtained insights into protein-induced conformational changes in DNA.
Quantified protein-induced B-Z transition dynamics of DNA.

Conclusions:

CSP-NMR titration is an effective method for studying protein-Z-DNA interactions.
The study provides a detailed analysis of protein-induced DNA conformational dynamics.
NMR titration data can elucidate the mechanisms of DNA binding proteins.