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A Multiparametric and High-Throughput Platform for Host-Virus Binding Screens.

Jan Schlegel1, Bartlomiej Porebski2, Luca Andronico1

  • 1Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, 17165 Solna, Sweden.

Nano Letters
|March 9, 2023
PubMed
Summary

This study introduces a high-throughput platform to rapidly identify host binding factors and antiviral drug targets during infectious disease outbreaks. The system accelerates the discovery of critical interactions, aiding in faster therapeutic development.

Keywords:
ACE2flow cytometrylipid bilayerneuropilin-1silica beadsvirus binding

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Area of Science:

  • Infectious Diseases
  • Virology
  • Drug Discovery

Background:

  • Rapid identification of host-pathogen interactions is crucial during infectious disease outbreaks.
  • The complexity of host plasma membranes hinders efficient screening for binding factors and drug targets.
  • Existing methods are often slow and lack high-throughput capabilities.

Purpose of the Study:

  • To develop and validate a multiparametric, high-throughput platform for accelerated identification of host binding factors.
  • To enable rapid screening for novel antiviral drug targets.
  • To overcome the limitations of current methods in studying host-pathogen interactions.

Main Methods:

  • Development of a novel multiparametric, high-throughput screening platform.
  • Utilizing the platform to analyze host-pathogen interactions.
  • Validation using SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) and human serum samples.

Main Results:

  • The platform successfully identified host binding factors and potential antiviral targets.
  • Demonstrated high sensitivity and robustness in screening assays.
  • Validated by blocking SARS-CoV-2 viral particles using nanobodies and immunoglobulins G (IgGs).

Conclusions:

  • The developed platform significantly accelerates the identification of host binding factors and antiviral targets.
  • This technology addresses a critical bottleneck in infectious disease research and drug development.
  • Offers a robust and sensitive tool for high-throughput screening against viral pathogens.