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Published on: January 7, 2019
A Multiparametric and High-Throughput Platform for Host-Virus Binding Screens
Jan Schlegel1, Bartlomiej Porebski2, Luca Andronico1
1Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, 17165 Solna, Sweden.
Abstract:
Speed is key during infectious disease outbreaks. It is essential, for example, to identify critical host binding factors to pathogens as fast as possible. The complexity of host plasma membrane is often a limiting factor hindering fast and accurate determination of host binding factors as well as high-throughput screening for neutralizing antimicrobial drug targets. Here, we describe a multiparametric and high-throughput platform tackling this bottleneck and enabling fast screens for host binding factors as well as new antiviral drug targets. The sensitivity and robustness of our platform were validated by blocking SARS-CoV-2 particles with nanobodies and IgGs from human serum samples.
Insights
This study introduces a high-throughput platform to rapidly identify host binding factors and antiviral drug targets during infectious disease outbreaks. The system accelerates the discovery of critical interactions, aiding in faster therapeutic development.
Area of Science:
- Infectious Diseases
- Virology
- Drug Discovery
Background:
- Rapid identification of host-pathogen interactions is crucial during infectious disease outbreaks.
- The complexity of host plasma membranes hinders efficient screening for binding factors and drug targets.
- Existing methods are often slow and lack high-throughput capabilities.
Purpose of the Study:
- To develop and validate a multiparametric, high-throughput platform for accelerated identification of host binding factors.
- To enable rapid screening for novel antiviral drug targets.
- To overcome the limitations of current methods in studying host-pathogen interactions.
Main Methods:
- Development of a novel multiparametric, high-throughput screening platform.
- Utilizing the platform to analyze host-pathogen interactions.
- Validation using SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) and human serum samples.
Main Results:
- The platform successfully identified host binding factors and potential antiviral targets.
- Demonstrated high sensitivity and robustness in screening assays.
- Validated by blocking SARS-CoV-2 viral particles using nanobodies and immunoglobulins G (IgGs).
Conclusions:
- The developed platform significantly accelerates the identification of host binding factors and antiviral targets.
- This technology addresses a critical bottleneck in infectious disease research and drug development.
- Offers a robust and sensitive tool for high-throughput screening against viral pathogens.
