5-Hydroxyeicosatetraenoate promotes Ca2+ and protein kinase C mobilization in neutrophils

5-Hydroxyeicosatetraenoate (5-HETE) stimulated human neutrophils to elevate their cytosolic Ca2+. It also enhanced the actions of phorbol myristate acetate and a diacylglycerol in stimulating these cells to: a) translocate protein kinase C from cytosol to membranes; b) generate superoxide anion; and c) release granule-bound enzymes. 5,20-DiHETE and 15-HETE possessed little or none of these bioactions. 5-HETE may influence neutrophil function by elevating cytosolic Ca2+ and thereby promoting the mobilization of protein kinase C.