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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Related Experiment Video

Updated: Aug 7, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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CRISPR-based biosensors for pathogenic biosafety.

Hao Yang1, Rodrigo Ledesma-Amaro2, Hong Gao1

  • 1College of Biomass Science and Engineering, Healthy Food Evaluation Research Center, Sichuan University, Chengdu, 610065, China.

Biosensors & Bioelectronics
|March 9, 2023
PubMed
Summary

CRISPR-Cas systems coupled with nanotechnology offer precise, rapid, and field-deployable tools for pathogen detection. These biosensors are crucial for advancing pathogenic biosafety and point-of-care diagnostics worldwide.

Keywords:
Drug-resistanceFungiParasitesPoint-of-careSARS-CoV-2 variantsViable bacteria

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Area of Science:

  • Biotechnology
  • Biosensing
  • Molecular Diagnostics

Background:

  • Pathogenic biosafety is a critical global concern requiring advanced analytical tools.
  • Existing methods for pathogen detection often lack the precision, speed, and field-deployability needed for immediate response.
  • Biotechnological innovations, particularly CRISPR/Cas systems, show promise for enhanced biosafety analysis.

Purpose of the Study:

  • To review the principles and applications of CRISPR/Cas systems in developing point-of-care (POC) biosensors for pathogen detection.
  • To highlight the potential of CRISPR-based technologies for rapid and accurate analysis of pathogenic threats.
  • To discuss the current challenges and future opportunities for CRISPR biosensors in pathogenic biosafety.

Main Methods:

  • Review of class II CRISPR/Cas system mechanisms for biomarker detection (nucleic acid and non-nucleic acid).
  • Summarization of molecular assays utilizing CRISPR technologies for POC applications.
  • Analysis of CRISPR tool applications in identifying various pathogens and their characteristics (genotype, phenotype, viability, drug-resistance).

Main Results:

  • CRISPR/Cas systems, when integrated with nanotechnologies, enable sensitive and specific detection of pathogens.
  • CRISPR-based assays can identify a wide range of pathogens, including bacteria, viruses, fungi, and parasites, along with their variants.
  • These systems facilitate the profiling of pathogen traits like viability and drug resistance, crucial for biosafety.

Conclusions:

  • CRISPR-based biosensors represent a significant advancement in achieving precise, rapid, and field-deployable pathogenic biosafety analysis.
  • The integration of CRISPR technology with nanotechnology offers a powerful platform for point-of-care diagnostics and global health security.
  • Further development is needed to overcome challenges and fully realize the potential of CRISPR biosensors in combating infectious diseases.