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Core genome multilocus sequence typing scheme for Bacillus cereus group bacteria.

Nicolas J Tourasse1, Keith A Jolley2, Anne-Brit Kolstø3

  • 1Department of Pharmacology and Pharmaceutical Biosciences, University of Oslo, Norway; University of Bordeaux, CNRS, INSERM, ARNA, UMR 5320, U1212, F-33000 Bordeaux, France.

Research in Microbiology
|March 9, 2023
PubMed
Summary

A new core genome multilocus sequence typing (cgMLST) scheme for the Bacillus cereus group uses 1568 core genes. This method offers enhanced resolution for phylogenetic analysis of these important bacterial species.

Keywords:
Bacillus cereus groupCore genomePhylogenyPubMLSTcgMLSTchewBBACA

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Area of Science:

  • Microbiology
  • Genomics
  • Bioinformatics

Background:

  • The Bacillus cereus group comprises several species, including human pathogens like B. cereus and B. anthracis, and the insect pathogen B. thuringiensis used in biopesticides.
  • Existing phylogenetic typing systems for the B. cereus group have limitations in resolution.
  • Accurate phylogenetic analysis is crucial for understanding the epidemiology and evolution of these bacteria.

Purpose of the Study:

  • To develop a high-resolution core genome multilocus sequence typing (cgMLST) scheme for the Bacillus cereus group.
  • To establish a standardized and publicly accessible typing system for B. cereus group bacteria.
  • To improve phylogenetic resolution compared to existing methods.

Main Methods:

  • Analysis of 173 complete B. cereus group genomes from public databases.
  • Identification of a core set of 1568 orthologous genes common to all members.
  • Implementation of the cgMLST scheme in the PubMLST system, creating an open online database.

Main Results:

  • A robust set of 1568 core genes was identified for cgMLST analysis of the B. cereus group.
  • The developed cgMLST scheme demonstrates superior resolution for phylogenetic analysis within the group.
  • The scheme is publicly available via the PubMLST database.

Conclusions:

  • The new cgMLST scheme provides an unprecedented level of resolution for phylogenetic studies of the Bacillus cereus group.
  • This tool will aid in the accurate classification, tracking, and understanding of B. cereus group bacteria.
  • The open-access nature of the PubMLST implementation promotes widespread community use and research.