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Related Experiment Video

Updated: Aug 7, 2025

High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize Zea mays L.
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Corn360: a method for quantification of corn kernels.

Samantha Gillette1, Lu Yin1, Penny M A Kianian2

  • 1School of Life Sciences, Arizona State University, Tempe, AZ, 85287, USA.

Plant Methods
|March 9, 2023
PubMed
Summary
This summary is machine-generated.

A new Corn360 system offers a portable, affordable way to phenotype corn kernels for yield estimation and genetic studies. This high-throughput method uses AI-powered software, reducing manual counting time and enabling detailed kernel trait analysis.

Keywords:
CornHigh-throughput phenotypingImage analysisKernel colorKernel textureLow-cost

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Area of Science:

  • Agricultural Science
  • Plant Breeding
  • Genetics

Background:

  • High-throughput phenotyping of corn kernel traits is crucial for yield estimation and genetic studies.
  • Existing methods often require complex setups, statistical expertise, and programming skills.

Purpose of the Study:

  • To develop a portable, accessible, and affordable system for high-throughput corn kernel phenotyping.
  • To enable accurate quantification of total and patterned corn kernel counts.
  • To facilitate the study of genetic inheritance of kernel traits.

Main Methods:

  • Developed Corn360, a panoramic imaging system for capturing corn ear images.
  • Utilized freely available software with Artificial Intelligence for image analysis and kernel segmentation.
  • Applied the system to homogeneously and mixed-patterned corn ears, including a sweet x sticky corn cross.

Main Results:

  • Corn360 achieved 93.7% accuracy for total kernel count on homogeneous ears, saving an average of 3 min 40 s per image.
  • Segmented kernel counts for mixed-patterned ears showed accuracies of 84.8% and 61.8%.
  • Demonstrated segregation of starch:sweet:sticky kernels in a 9:4:3 ratio in an F2 population, indicating control by two genes with epistatic effects.

Conclusions:

  • The Corn360 system provides a portable, low-cost, high-throughput solution for corn kernel quantification.
  • Enables rapid yield component estimation and categorization of patterned kernels for genetic studies.
  • Corn360 is effective, accessible, and cost-efficient for corn kernel analysis, regardless of programming skills.