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Clostridioides difficile Binary Toxin Binding Component Increases Virulence in a Hamster Model.

Morgan Simpson1, Terry Bilverstone2, Jhansi Leslie3

  • 1Department of Pathology, University of Virginia, Charlottesville, Virginia, USA.

Open Forum Infectious Diseases
|March 10, 2023
PubMed
Summary
This summary is machine-generated.

The binding component of Clostridioides difficile binary toxin (CDT), CDTb, drives severe illness in hamsters. This finding clarifies CDT

Keywords:
CDTClostridioides difficilebinary toxinhamster model

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Toxicology

Background:

  • Clostridioides difficile is a major cause of hospital-acquired infections.
  • Hypervirulent strains expressing binary toxin (CDT) contribute to C. difficile pathogenesis.
  • The role of individual CDT components in vivo remains unclear.

Purpose of the Study:

  • To elucidate the specific contribution of CDTa and CDTb to C. difficile virulence.
  • To investigate the in vivo function of individual CDT components during infection.

Main Methods:

  • Generated C. difficile strains expressing either CDTa or CDTb alone.
  • Infected mouse and hamster models with engineered C. difficile strains.
  • Monitored disease progression and severity in infected animals.

Main Results:

  • CDTb expression alone did not cause significant disease in mice.
  • CDTb alone restored virulence in a hamster model of C. difficile infection.
  • CDT-deficient C. difficile complemented with CDTb alone was virulent in hamsters.

Conclusions:

  • The binding component of C. difficile binary toxin, CDTb, is a key virulence factor.
  • CDTb contributes significantly to C. difficile pathogenesis in a hamster model.
  • Understanding individual toxin component roles is crucial for combating hypervirulent strains.