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Updated: Aug 7, 2025

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
Published on: May 1, 2015
From lymphatic endothelial cell migration to formation of tubular lymphatic vascular network
Tomohiro Shiiya1, Masanori Hirashima1
1Division of Pharmacology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
Abstract:
During development, lymphatic endothelial cell (LEC) progenitors differentiate from venous endothelial cells only in limited regions of the body. Thus, LEC migration and subsequent tube formation are essential processes for the development of tubular lymphatic vascular network throughout the body. In this review, we discuss chemotactic factors, LEC-extracellular matrix interactions and planar cell polarity regulating LEC migration and formation of tubular lymphatic vessels. Insights into molecular mechanisms underlying these processes will help in understanding not only physiological lymphatic vascular development but lymphangiogenesis associated with pathological conditions such as tumors and inflammation.
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