Related Experiment Video
Updated: Aug 1, 2026

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy
Published on: May 22, 2020
Variations in the flow of cerebrospinal fluid through spinal needles
1Department of Anaesthetics, Northern General Hospital, Sheffield.
Spinal needle dimensions and performance varied significantly across available products, deviating from international standards. This variability impacts the reliability of cerebrospinal fluid collection during medical procedures.
Area of Science:
- Medical Devices
- Anesthesiology
- Neurosurgery
Background:
- Spinal needles are critical for accessing cerebrospinal fluid.
- Standardization of spinal needle dimensions is essential for consistent performance.
- Existing spinal needles vary in external diameter from 0.45 mm (26-gauge) to 0.7 mm (22-gauge).
Purpose of the Study:
- To measure the dimensions of available spinal needles.
- To compare these dimensions against the International Standard (ISO 7864-1984 E).
- To assess the in vitro performance of spinal needles regarding cerebrospinal fluid appearance time and flow rates.
Main Methods:
- Dimensional analysis of multiple spinal needle types.
- In vitro testing to measure cerebrospinal fluid (CSF) dynamics.
- Comparison of measured parameters with ISO 7864-1984 E standards.
Main Results:
- Significant variations were observed in the external diameters of tested spinal needles.
- Performance metrics, including time to CSF appearance and flow rates, showed considerable differences.
- Deviations from the International Standard (ISO 7864-1984 E) were noted in needle dimensions.
Conclusions:
- Current spinal needles exhibit substantial variability in dimensions and in vitro performance.
- This heterogeneity may affect procedural outcomes and patient safety.
- Adherence to international standards for spinal needles requires further investigation and potential revision.
Related Concept Videos
Local Anesthetics: Clinical Application as Spinal Anesthesia
Anatomy of the Brain: Ventricles
Cerebrospinal Fluid
CSF Production
CSF is produced mainly in the choroid plexus, a network of capillaries and ependymal cells located within the ventricular system of the brain.
Increased Intracranial Pressure l: Introduction
Cerebral Edema l: Introduction
Cerebral Edema ll: Pathophysiology

