Related Experiment Video
Updated: Aug 7, 2025
07:15
A Mouse Model of Incompletely Resected Soft Tissue Sarcoma for Testing Neoadjuvant Therapies
Published on: July 28, 2020
9.7K
Tumor Suppression by PD-1/PD-L1 Interaction Blockage in Mice Model
Shima Salehi1, Hajarossadat Ghaderi1, Mahdi Habibi-Anbouhi2
1Biotechnology Research Center, Venom and Biotherapeutics Molecules Laboratory, Pasteur Institute of Iran, Tehran, Iran.
Iranian Journal of Pharmaceutical Research : IJPR
|March 10, 2023
Summary
Blocking the interaction between programmed cell death protein 1 (PD-1) and programmed cell death ligand 1 (PD-L1) with recombinant PD-1 effectively reduced tumor growth and improved survival rates in mice.
Area of Science:
- Immunology
- Oncology
- Biotechnology
Background:
- Tumor cells evade immune detection via PD-L1/PD-1 interactions, suppressing T-cell activity.
- Targeting this pathway offers a strategy to restore anti-tumor immunity.
Purpose of the Study:
- To investigate the therapeutic potential of a recombinant mouse PD-1 (mPD-1) protein.
- To assess its ability to block PD-L1 and inhibit tumor growth.
Main Methods:
- Recombinant mPD-1 was expressed in E. coli and purified.
- Binding affinity to human PD-L1 was confirmed via ELISA.
- Antitumor efficacy was evaluated in tumor-bearing mice.
Main Results:
- mPD-1 demonstrated significant binding to human PD-L1.
- Intra-tumoral mPD-1 injections led to decreased tumor size.
- Survival rates increased, and tumor necrosis was observed.
Conclusions:
- Blocking the PD-1/PD-L1 interaction is a viable strategy for targeted cancer therapy.
- Recombinant PD-1 shows promise as an anti-tumor agent.
Related Concept Videos
02:43Mouse Models of Cancer Study
5.6K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
5.6K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
01:27Tumor Immunotherapy
606
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
606