A turn-on NIR fluorescent probe for risk-assessing oxidative stress in cabbage roots under abiotic stress

Oxidative stress is closely related to the crop health status under stress conditions. H₂O₂ is an important signaling molecule in plants under stress. Therefore, monitoring H₂O₂ fluctuations is of great significance when risk-assessing oxidative stress. However, few fluorescent probes have been reported for the in situ tracking of H₂O₂ fluctuations in crops. Herein, we designed a "turn-on" NIR fluorescent probe (DRP-B) to detect and in situ-image H₂O₂ in living cells and crops. DRP-B exhibited good detection performance for H₂O₂ and could image endogenous H₂O₂ in living cells. More importantly, it could semi-quantitatively visualize H₂O₂ in cabbage roots under abiotic stress. Visualization of H₂O₂ in cabbage roots revealed H₂O₂ upregulation in response to adverse environments (metals, flood, and drought). This study provides a new method for risk-assessing oxidative stress in plants under abiotic stress and is expected to provide guidance for the development of new antioxidant defense strategies to enhance plant resistance and crop productivity.