Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

1.8K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
1.8K
T Cell Types and Functions01:24

T Cell Types and Functions

1.2K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.2K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

69.5K
Overview
69.5K
Special Features of Adaptive Immunity01:20

Special Features of Adaptive Immunity

954
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
954
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

1.1K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
1.1K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

2.0K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ITK Deficiency Attenuates Alveolar Hemorrhage by Enhancing Regulatory T Cell-Mediated Tissue Resilience.

bioRxiv : the preprint server for biology·2026
Same author

β-Catenin stabilization protects against alveolar hemorrhage through amphiregulin- and BATF-mediated Tregs.

JCI insight·2026
Same author

TCF1 dosage determines cell fate during T cell development.

Science advances·2024
Same author

Retraction Note: Aging exacerbates the brain inflammatory micro-environment contributing to α-synuclein pathology and functional deficits in a mouse model of DLB/PD.

Molecular neurodegeneration·2024
Same author

Successful Treatment of Plasma Cell-Rich Acute Rejection Using Bortezomib: A Case Report.

Case reports in transplantation·2024
Same author

Discrepancy Analysis between Histology and Molecular Diagnoses in Kidney Allograft Biopsies: A Single-Center Experience.

International journal of molecular sciences·2023
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Aug 7, 2025

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
09:04

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Published on: April 19, 2017

13.6K

TCF-1 Is Required for CD4 T Cell Persistence Functions during AlloImmunity.

Mahinbanu Mammadli1, Liye Suo2, Jyoti Misra Sen3,4

  • 1Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, NY 13210, USA.

International Journal of Molecular Sciences
|March 11, 2023
PubMed
Summary
This summary is machine-generated.

Transcription factor T cell factor-1 (TCF-1) is crucial for mature CD4 T cell stemness and persistence. Loss of TCF-1 prevents graft-versus-host disease, highlighting its role in alloimmunity.

Keywords:
CD4 T cell serum level cytokine productionCD4 T cells stemnessTCF-1alloimmunity

More Related Videos

Generation of Human Alloantigen-specific T Cells from Peripheral Blood
09:47

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Published on: November 21, 2014

13.1K
Author Spotlight: Elucidating the Pathways of TFH Cell Differentiation in Acute LCMV Challenges
05:03

Author Spotlight: Elucidating the Pathways of TFH Cell Differentiation in Acute LCMV Challenges

Published on: April 26, 2024

746

Related Experiment Videos

Last Updated: Aug 7, 2025

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
09:04

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Published on: April 19, 2017

13.6K
Generation of Human Alloantigen-specific T Cells from Peripheral Blood
09:47

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Published on: November 21, 2014

13.1K
Author Spotlight: Elucidating the Pathways of TFH Cell Differentiation in Acute LCMV Challenges
05:03

Author Spotlight: Elucidating the Pathways of TFH Cell Differentiation in Acute LCMV Challenges

Published on: April 26, 2024

746

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • The transcription factor T cell factor-1 (TCF-1) is vital for immune responses.
  • TCF-1's role in CD4 T cell development is established, but its function in mature peripheral CD4 T cell-mediated alloimmunity remains unclear.

Purpose of the Study:

  • To investigate the biological function of TCF-1 in mature peripheral CD4 T cell-mediated alloimmunity.
  • To elucidate the mechanisms by which TCF-1 regulates CD4 T cell stemness, persistence, and effector functions.

Main Methods:

  • Utilized TCF-1 conditional knockout (cKO) mouse models.
  • Performed allogeneic CD4 T cell transplantation experiments.
  • Conducted transcriptomic analysis to uncover regulated pathways.

Main Results:

  • TCF-1 is critical for mature CD4 T cell stemness and persistence.
  • Mature CD4 T cells from TCF-1 cKO mice did not induce graft-versus-host disease (GvHD).
  • TCF-1 regulates CD4 T cell stemness via CD28 expression, effector/memory formation, and chemokine/cytokine receptor expression.

Conclusions:

  • TCF-1 plays a critical role in regulating mature CD4 T cell-mediated alloimmunity.
  • TCF-1 controls CD4 T cell stemness, persistence, and inflammatory responses.
  • Understanding TCF-1's function provides a basis for developing targeted therapies for CD4 T cell-mediated diseases.