Sphingolipid Long-Chain Base Signaling in Compatible and Non-Compatible Plant-Pathogen Interactions in Arabidopsis.
Mariana Saucedo-García1, Ariadna González-Solís2, Priscila Rodríguez-Mejía3
1Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Tulancingo 43600, Mexico.
International Journal of Molecular Sciences
|March 11, 2023
Summary
Plant sphingolipids, specifically long-chain bases (LCBs), act as signaling molecules. A pathway involving LCBs, mitogen-activated protein kinase 6 (MPK6), and reactive oxygen species (ROS) enhances plant immunity against specific pathogens.
Area of Science:
- Plant biology
- Molecular signaling
- Plant immunity
Background:
- Sphingolipids in plants exhibit diverse roles, including signaling functions for long-chain bases (LCBs).
- LCB signaling is linked to plant immunity, involving mitogen-activated protein kinase 6 (MPK6) and reactive oxygen species (ROS).
Purpose of the Study:
- To investigate the role of LCBs, MPK6, and ROS in plant defense mechanisms.
- To elucidate the signaling pathway connecting LCBs to plant immunity.
Main Methods:
- Utilized in planta assays with mutants and fumonisin B1 (FB1) to manipulate sphingolipid levels.
- Conducted pathogenicity tests using virulent and avirulent *Pseudomonas syringae* strains.
Main Results:
- FB1 or avirulent strains induced a surge in free LCBs and ceramides, triggering biphasic ROS production.
- The first ROS phase involved NADPH oxidase; the second, sustained phase related to programmed cell death.
- MPK6 acted downstream of LCB buildup and upstream of late ROS, inhibiting avirulent strain growth.
Conclusions:
- A LCB-MPK6-ROS signaling pathway contributes to plant immunity.
- This pathway differentially regulates defense responses, enhancing immunity against non-compatible interactions.
Keywords:
effector-triggered immunity (ETI)fumonisin B1 (FB1)long-chain bases (LCBs)mitogen-activated protein kinase 6 (MPK6)non-compatible interactionpathogen plant defenseplant diseaseprogrammed cell death (PCD)reactive oxygen species (ROS)sphingolipidsMore Related Videos
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