Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oligosaccharide Assembly01:24

Oligosaccharide Assembly

2.9K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
2.9K
Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

46
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
46
Cellulose and Pectic Polysaccharides01:15

Cellulose and Pectic Polysaccharides

3.7K
 Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...
3.7K
Diversity of Protists IV01:27

Diversity of Protists IV

68
Amoebozoa represent a diverse group of terrestrial and aquatic protists that utilize lobe-shaped pseudopodia for locomotion and feeding. This characteristic differentiates them from the Rhizaria, which possess threadlike pseudopodia. The primary classifications within Amoebozoa include gymnamoebas, entamoebas, and the plasmodial and cellular slime molds. Phylogenetic evidence indicates that Amoebozoa diverged from a lineage that ultimately gave rise to fungi and animals.Gymnamoebas and...
68
Diversity of Protists III01:27

Diversity of Protists III

78
Rhizaria are a diverse group of unicellular protists characterized by their threadlike cytoplasmic extensions known as pseudopodia. These structures aid in both locomotion and feeding, giving Rhizaria an amoeboid appearance. Their amoeboid morphology once led to taxonomic confusion, but molecular phylogenetics has clarified their evolutionary placement and emphasized their shared use of pseudopodia despite divergent lineages.This clade comprises diverse lineages such as Chlorarachniophyta,...
78
Diversity of Protists II01:27

Diversity of Protists II

79
Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...
79

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unravelling the potential of yolkin for nutraceutical use: the origin, structure, and functional insights of a hen egg yolk polypeptide complex.

Food & function·2024
Same author

New Structure of <i>Aeromonas salmonicida</i> O-Polysaccharide Isolated from Ill Farmed Fish.

Microorganisms·2024
Same author

Characteristics of Environmental <i>Klebsiella pneumoniae</i> and <i>Klebsiella oxytoca</i> Bacteriophages and Their Therapeutic Applications.

Pharmaceutics·2023
Same author

Multi-Omic Profiling of a Newly Isolated Oxy-PAH Degrading Specialist from PAH-Contaminated Soil Reveals Bacterial Mechanisms to Mitigate the Risk Posed by Polar Transformation Products.

Environmental science & technology·2022
Same author

Complete Characterization of the O-Antigen from the LPS of <i>Aeromonas bivalvium</i>.

International journal of molecular sciences·2022
Same author

Roles of Proteins Containing Immunoglobulin-Like Domains in the Conjugation of Bacterial Plasmids.

mSphere·2022

Related Experiment Video

Updated: Aug 7, 2025

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques
11:49

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques

Published on: March 24, 2016

7.7K

Structural Diversity among Edwardsiellaceae Core Oligosaccharides.

Maria Jordán1, Sylwia Wojtys-Tekiel2, Susana Merino1

  • 1Department of Genetic, Microbiology and Statistic, University of Barcelona, Diagonal 643, 08028 Barcelona, Spain.

International Journal of Molecular Sciences
|March 11, 2023
PubMed
Summary

This study reveals the chemical structures and gene functions of lipopolysaccharide (LPS) core oligosaccharides in four pathogenic *Edwardsiella* species. Understanding these structures is key to developing new treatments for bacterial infections in fish and humans.

Keywords:
EdwardsiellaeaNMR spectroscopycore oligosaccharidegenomic

More Related Videos

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
09:56

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

6.9K
Improved In-gel Reductive &#946;-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
13:06

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Published on: November 20, 2014

11.9K

Related Experiment Videos

Last Updated: Aug 7, 2025

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques
11:49

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques

Published on: March 24, 2016

7.7K
Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
09:56

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

6.9K
Improved In-gel Reductive &#946;-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
13:06

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Published on: November 20, 2014

11.9K

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Structural Biology

Background:

  • The *Edwardsiella* genus comprises five pathogenic species, including *E. tarda*, *E. anguillarum*, *E. piscicida*, *E. hoshinae*, and *E. ictaluri*.
  • These bacteria cause significant infections in fish and can also affect reptiles, birds, and humans.
  • Lipopolysaccharide (LPS), an endotoxin, is a critical factor in the pathogenesis of *Edwardsiella* infections.

Purpose of the Study:

  • To elucidate the chemical structures of lipopolysaccharide (LPS) core oligosaccharides in *E. piscicida*, *E. anguillarum*, *E. hoshinae*, and *E. ictaluri* for the first time.
  • To identify and assign the genes responsible for the biosynthesis of these core oligosaccharides.
  • To provide a foundation for understanding LPS-mediated virulence and developing targeted interventions.

Main Methods:

  • Genomic analysis to identify genes involved in core LPS biosynthesis.
  • Nuclear Magnetic Resonance (NMR) spectroscopy (¹H and ¹³C) to determine the detailed chemical structures of core oligosaccharides.
  • Comparative structural analysis across different *Edwardsiella* species.

Main Results:

  • Complete gene assignments for core biosynthesis functions were obtained for the studied species.
  • *E. piscicida* and *E. anguillarum* share similar core oligosaccharide structures, featuring specific heptose, glucose, glucNAc, galactose, and Kdo residues.
  • *E. hoshinae* and *E. ictaluri* exhibit distinct core structures with variations in terminal sugars and the presence/absence of specific components like α-D-GlcNAc and α-D-GlcNAc.

Conclusions:

  • The study provides the first detailed chemical structures and genomic insights into the LPS core oligosaccharides of four key *Edwardsiella* species.
  • Significant structural variations exist in the LPS core among these closely related pathogens, potentially influencing their host interactions and virulence.
  • These findings are crucial for understanding *Edwardsiella* pathogenesis and for developing novel strategies against infections caused by these bacteria.