Organization of the nigrotectospinal pathway in the cat: a light and electron microscopic study

The relationships between nigrotectal terminals and tectospinal neurons in the deep layers of the superior colliculus have been examined light and electron microscopically by using antero- and retrograde transport of tracer, and anterograde degeneration. For light microscopy, horseradish peroxidase (HRP) was injected into the cervical spinal cord, and HRP conjugated to wheat germ agglutinin (WGA-HRP) into the substantia nigra at the same time. It appeared that about 22% of the retrogradely labeled tectospinal neurons were totally or partially in the anterogradely labeled nigral terminal clusters. This coexistence was particularly manifest in the dorso-lateral part of the intermediate gray layer. For electron microscopy, nigral lesions and HRP injections into the cervical spinal cord were combined in the same animals. Degenerating nigrotectal terminals were found in both axosomatic and axodendritic symmetrical synaptic contacts with retrogradely labeled tectospinal neurons in the intermediate gray layer. Present experiments show tectospinal neurons receiving monosynaptic input from the substantia nigra do exist in the intermediate gray layer of the superior colliculus, especially in its dorsolateral portion.