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Updated: Aug 7, 2025

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

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Transgene Bioconfinement: Don't Flow There.

Jessica N Stockdale¹, Reginald J Millwood¹

¹Department of Plant Sciences, University of Tennessee (UTK), Knoxville, TN 37996, USA.

Plants (Basel, Switzerland)

|March 11, 2023

Summary

This summary is machine-generated.

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Genetically engineered (GE) crops offer benefits but raise concerns about transgene spread. Bioconfinement systems aim to prevent this, with various methods surveyed for efficacy and adoption potential.

Area of Science:

Agricultural Science
Genetics
Environmental Science

Background:

Genetically engineered (GE) crops provide economic and environmental advantages.
Concerns exist regarding transgene movement from GE crops to wild relatives, especially in native regions.
Enhanced fitness traits in GE crops could negatively impact natural populations if introgressed.

Purpose of the Study:

To survey bioconfinement systems for preventing transgene flow from GE crops.
To evaluate the utility, efficacy, and commercial adoption potential of various bioconfinement strategies.
To discuss the role of emerging technologies like CRISPR/Cas9 in transgene containment.

Main Methods:

Review of existing and experimental bioconfinement strategies.

Keywords:

GE crops bioconfinement delayed flowering gene flow male sterility transgene excision transgenic plants

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Analysis of systems focusing on male and seed sterility.

Assessment of transgene excision, delayed flowering, and gene editing approaches.

Main Results:

Several bioconfinement approaches show promise for preventing transgene flow.
No single system has achieved widespread commercial adoption despite decades of GE crop use.
CRISPR/Cas9 technology presents a potential avenue for reducing or eliminating transgene flow.

Conclusions:

Bioconfinement systems may be necessary for new GE crops or those with high outcrossing potential.
Further development and evaluation are needed to ensure system efficacy and commercial viability.
Addressing regulatory and environmental concerns requires robust transgene containment solutions.