Silver Inhibits Lemna minor Growth at High Initial Frond Densities

Silver nanoparticles (AgNPs) are the most popular engineered nanomaterials in consumer products due to their antimicrobial properties. They enter aquatic ecosystems via insufficient purified wastewaters from manufacturers or consumers. AgNPs inhibit growth of aquatic plants, including duckweeds. Growth media nutrient concentration and initial duckweed frond density can affect growth. However, it is not well understood how frond density affects nanoparticle toxicity. We investigated the toxicity of 500 µg/L AgNPs and AgNO₃ on Lemna minor at different initial frond densities (20, 40, and 80 fronds per 28.5 cm²) over 14 days. Plants were more sensitive to silver at high initial frond densities. Growth rates based on frond number and area were lower for plants at 40 and 80 initial frond density in both silver treatments. AgNPs had no effect on frond number, biomass, and frond area at 20 initial frond density. However, AgNO₃ plants had lower biomass than control and AgNP plants at 20 initial frond density. Competition and crowding at high frond densities resulted in reduced growth when silver was present, therefore plant density and crowding effects should be considered in toxicity studies.