Cellulosic Ethanol Production from Weed Biomass Hydrolysate of Vietnamosasa pusilla

Lignocellulosic biomass can be used as a renewable and sustainable energy source to help reduce the consequences of global warming. In the new energy age, the bioconversion of lignocellulosic biomass into green and clean energy displays remarkable potential and makes efficient use of waste. Bioethanol is a biofuel that can diminish reliance on fossil fuels while minimizing carbon emissions and increasing energy efficiency. Various lignocellulosic materials and weed biomass species have been selected as potential alternative energy sources. Vietnamosasa pusilla, a weed belonging to the Poaceae family, contains more than 40% glucan. However, research on the applications of this material is limited. Thus, here we aimed to achieve maximum fermentable glucose recovery and bioethanol production from weed biomass (V. pusilla). To this end, V. pusilla feedstocks were treated with varying concentrations of H₃PO₄ and then subjected to enzymatic hydrolysis. The results indicated that after pretreatment with different concentrations of H₃PO₄, the glucose recovery and digestibility at each concentration were markedly enhanced. Moreover, 87.5% of cellulosic ethanol was obtained from V. pusilla biomass hydrolysate medium without detoxification. Overall, our findings reveal that V. pusilla biomass can be introduced into sugar-based biorefineries to produce biofuels and other valuable chemicals.