Related Experiment Video
Updated: Aug 7, 2025
08:00
Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study
Published on: April 11, 2018
10.7K
Potentiometric Chloride Ion Biosensor for Cystic Fibrosis Diagnosis and Management: Modeling and Design
Annabella la Grasta1, Martino De Carlo1, Attilio Di Nisio1
1Department of Electrical and Information Engineering, Polytechnic University of Bari, 70125 Bari, Italy.
Sensors (Basel, Switzerland)
|March 11, 2023
Summary
This study introduces an ion-sensitive field-effect transistor for detecting chloride ions in sweat, aiding cystic fibrosis diagnosis. The novel device offers a cost-effective, non-invasive alternative to traditional sweat tests.
Area of Science:
- Biomedical Engineering
- Materials Science
- Analytical Chemistry
Background:
- Ion-sensitive field-effect transistors (ISFETs) are established for pH sensing.
- Expanding ISFET applications to detect other biomarkers in biological fluids for medical use is an active research area.
Purpose of the Study:
- To develop and validate an ISFET capable of detecting chloride ions in sweat.
- To assess the device's potential for diagnosing cystic fibrosis.
Main Methods:
- Utilized the finite element method to model the semiconductor and electrolyte domains of the ISFET.
- Investigated the chemical interactions between the gate oxide and the electrolytic solution.
Main Results:
- Achieved a limit-of-detection of 0.004 mol/m³ for chloride ions in sweat.
- Demonstrated that anions interact with hydroxyl surface groups, replacing adsorbed protons.
Conclusions:
- The developed ISFET is suitable for detecting chloride ions in sweat, supporting cystic fibrosis diagnosis.
- This technology presents an easy-to-use, cost-effective, and non-invasive alternative to conventional sweat tests, enabling earlier and more accurate diagnoses.
Related Concept Videos
01:23Cystic Fibrosis: Pathogenesis
320
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
320
01:24Cystic Fibrosis: Management
208
Cystic fibrosis (CF) is an autosomal recessive disorder that predominantly affects individuals of Northern European descent, occurring at a rate of 1 in 3500. It is caused by a genetic mutation in a gene on chromosome 7, most commonly the ΔF508 mutation, that codes for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. This results in thicker mucus secretions and obstruction pathologies in multiple organs, including the lungs and sinuses.
Sinus disease and chronic...
Sinus disease and chronic...
208
01:15Potentiometry: Membrane Electrodes
663
Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
663